Structure of RyR1 in native membranes
- Ryanodine receptor 1 (RyR1) mediates excitation–contraction coupling by releasing Ca2+ from sarcoplasmic reticulum (SR) to the cytoplasm of skeletal muscle cells. RyR1 activation is regulated by several proteins from both the cytoplasm and lumen of the SR. Here, we report the structure of RyR1 from native SR membranes in closed and open states. Compared to the previously reported structures of purified RyR1, our structure reveals helix‐like densities traversing the bilayer approximately 5 nm from the RyR1 transmembrane domain and sarcoplasmic extensions linking RyR1 to a putative calsequestrin network. We document the primary conformation of RyR1 in situ and its structural variations. The activation of RyR1 is associated with changes in membrane curvature and movement in the sarcoplasmic extensions. Our results provide structural insight into the mechanism of RyR1 in its native environment.
| Author: | Wenbo ChenGND, Mikhail KudryashevORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-564403 |
| DOI: | https://doi.org/10.15252/embr.201949891 |
| ISSN: | 1469-3178 |
| Parent Title (English): | EMBO report |
| Publisher: | Wiley |
| Place of publication: | Hoboken, NJ [u.a.] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/03/09 |
| Date of first Publication: | 2020/03/09 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/10/28 |
| Tag: | cryo-electron tomography; muscle contraction; ryanodine receptor; sarcoplasmic reticulum; subtomogram averaging |
| Volume: | 21 |
| Issue: | 5, art. e49891 |
| Page Number: | 11 |
| HeBIS-PPN: | 472248413 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
