Structure validation of G‐rich RNAs in noncoding regions of the human genome
- We present the rapid biophysical characterization of six previously reported putative G‐quadruplex‐forming RNAs from the 5′‐untranslated region (5′‐UTR) of silvestrol‐sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]2[CGG]2C—folds into a single well‐defined G‐quadruplex structure. Sequences with longer poly‐G strands form unspecific aggregates, whereas CGG‐repeat‐containing sequences exhibit a temperature‐dependent equilibrium between a hairpin and a G‐quadruplex structure. The applied experimental strategy is fast and provides robust readout for G‐quadruplex‐forming capacities of RNA oligomers.
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| Author: | Oliver BinasORCiDGND, Irene BessiORCiDGND, Harald SchwalbeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-564929 |
| DOI: | https://doi.org/10.1002/cbic.201900696 |
| ISSN: | 1439-7633 |
| ISSN: | 1439-4227 |
| Parent Title (German): | ChemBioChem |
| Publisher: | Wiley-VCH Verlag GmbH & Co. KGaA. |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2020/01/14 |
| Date of first Publication: | 2020/01/14 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2020/10/28 |
| Tag: | G-quadruplexes; NMR spectroscopy; RNA; biophysical investigation; circular dichroism |
| Volume: | 21 |
| Issue: | Issue Horst Kessler’s 80th Birthday |
| Page Number: | 8 |
| First Page: | 1656 |
| Last Page: | 1663 |
| HeBIS-PPN: | 471418374 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
