Complexome profiling: assembly and remodeling of protein complexes

  • Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease.

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Author:Ilka WittigORCiD, Pedro Felipe Malacarne
URN:urn:nbn:de:hebis:30:3-618958
DOI:https://doi.org/10.3390/ijms22157809
ISSN:1422-0067
Parent Title (English):International journal of molecular sciences
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2021/07/21
Date of first Publication:2021/07/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/07/30
Tag:assembly; complexome profiling; data repositories; mass spectrometry; protein complexes; protein–protein interaction; remodeling
Volume:22
Issue:15, art. 7809
Page Number:12
First Page:1
Last Page:12
HeBIS-PPN:487211847
Institutes:Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0