Autophagy-related activation of hepatic stellate cells reduces cellular miR-29a by promoting its vesicular secretion

  • Background & Aims: Liver fibrosis arises from long-term chronic liver injury, accompanied by an accelerated wound healing response with interstitial accumulation of extracellular matrix (ECM). Activated hepatic stellate cells (HSC) are the main source for ECM production. MicroRNA29a (miR-29a) is a crucial antifibrotic miRNA that is repressed during fibrosis, resulting in up-regulation of collagen synthesis. Methods; Intracellular and extracellular miRNA levels of primary and immortalized myofibroblastic HSC in response to profibrogenic stimulation by transforming growth factor β (TGFβ) or platelet-derived growth factor-BB (PDGF-BB) or upon inhibition of vesicular transport and autophagy processes were determined by quantitative polymerase chain reaction. Autophagy flux was studied by electron microscopy, flow cytometry, immunoblotting, and immunocytochemistry. Hepatic and serum miR-29a levels were quantified by using both liver tissue and serum samples from a cohort of chronic hepatitis C virus patients and a murine CCl4 induced liver fibrosis model. Results: In our study, we show that TGFβ and PDGF-BB resulted in decrease of intracellular miR-29a and a pronounced increase of vesicular miR-29a release into the supernatant. Strikingly, miR-29a vesicular release was accompanied by enhanced autophagic activity and up-regulation of the autophagy marker protein LC3. Moreover, autophagy inhibition strongly prevented miR-29a secretion and repressed its targets’ expression such as Col1A1. Consistently, hepatic miR-29a loss and increased LC3 expression in myofibroblastic HSC were associated with increased serum miR-29a levels in CCl4-treated murine liver fibrosis and specimens of hepatitis C virus patients with chronic liver disease. Conclusions: We provide evidence that activation-associated autophagy in HSC induces release of miR-29a, whereas inhibition of autophagy represses fibrogenic gene expression in part through attenuated miR-29a secretion.

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Author:Xiaojie YuORCiDGND, Natalia ElfimovaGND, Marion Müller, Daniel BachurskiORCiD, Ulrike Koitzsch, Uta DrebberGND, Esther Mahabir-BrennerORCiDGND, Hinrich P. Hansen, Scott L. FriedmanORCiDGND, Sabine Klein, Hans Peter DienesGND, Marianna HöselORCiD, Reinhard BüttnerGND, Jonel TrebickaORCiDGND, Vangelis KondylisORCiDGND, Inge MannaertsORCiD, Margarete OdenthalORCiDGND
URN:urn:nbn:de:hebis:30:3-631082
DOI:https://doi.org/10.1016/j.jcmgh.2022.02.013
ISSN:2352-345X
Parent Title (English):Cellular and Molecular Gastroenterology and Hepatology
Publisher:Elsevier
Place of publication:New York, NY
Document Type:Article
Language:English
Date of Publication (online):2022/04/21
Date of first Publication:2022/04/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/11/28
Tag:Circulating MiRNA; Liver Fibrosis; MiR-29
Volume:13
Issue:6
Page Number:16
First Page:1701
Last Page:1716
Institutes:Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International