Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo‐tomography
- We employed electron cryo‐tomography to visualize cytosolic ribosomes on the surface of mitochondria. Translation‐arrested ribosomes reveal the clustered organization of the TOM complex, corroborating earlier reports of localized translation. Ribosomes are shown to interact specifically with the TOM complex, and nascent chain binding is crucial for ribosome recruitment and stabilization. Ribosomes are bound to the membrane in discrete clusters, often in the vicinity of the crista junctions. This interaction highlights how protein synthesis may be coupled with transport. Our work provides unique insights into the spatial organization of cytosolic ribosomes on mitochondria.
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| Author: | Vicki A. M. GoldORCiD, Piotr Chroscicki, Piotr Bragoszewski, Agnieszka Chacinska |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-465779 |
| DOI: | https://doi.org/10.15252/embr.201744261 |
| ISSN: | 1469-3178 |
| ISSN: | 1469-221X |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/28827470 |
| Parent Title (English): | EMBO reports |
| Publisher: | EMBO Press ; Wiley |
| Place of publication: | Heidelberg ; Hoboken, NJ [u. a.] |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2017 |
| Date of first Publication: | 2017/08/21 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2018/06/07 |
| Tag: | TOM complex; electron cryo-tomography; mitochondrial protein import; ribosomes; translation |
| Volume: | 18 |
| Issue: | 10 |
| Page Number: | 15 |
| First Page: | 1786 |
| Last Page: | 1800 |
| Note: | License: This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| HeBIS-PPN: | 434433993 |
| Institutes: | Biowissenschaften / Biowissenschaften |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Sammlung Biologie / Sondersammelgebiets-Volltexte | |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
