Refine
Document Type
- Article (14)
- Doctoral Thesis (1)
Language
- English (15)
Has Fulltext
- yes (15)
Is part of the Bibliography
- no (15)
Keywords
- Machine learning (2)
- Penile carcinomas (2)
- Prostate cancer (2)
- classical Hodgkin lymphoma (2)
- tumor microenvironment (2)
- Antibodies (1)
- Artificial intelligence (1)
- Caco-2 cells (1)
- Cell motility (1)
- Colorectal cancer (1)
- Cytoskeletal proteins (1)
- HPV infection (1)
- Immunohistochemistry techniques (1)
- LCD-Array (1)
- Metastasis (1)
- Metastatic tumors (1)
- Multiparametric MRI (1)
- Paediatric cancer (1)
- Prediction (1)
- Quantitative features (1)
- Radiomics (1)
- SW480 cells (1)
- Sanger Sequencing (1)
- Screening (1)
- Statistical analysis (1)
- Tissue microarray (1)
- Tumor microenvironment (1)
- Tumour immunology (1)
- Urological oncology (1)
- Viral infection (1)
- Viral oncology (1)
- adjuvant chemotherapy (1)
- bladder cancer (1)
- breast cancer (1)
- cancer associated fibroblasts (1)
- computational pathology (1)
- consensus classification (1)
- deep learning (1)
- diffusion-weighted magnetic resonance imaging (1)
- double negative (1)
- ensemble cNN (1)
- fibroblasts (1)
- flow cytometry (1)
- gene expression analysis (1)
- histology (1)
- immunohistochemistry (1)
- luteolin (1)
- macrophage (1)
- methylation profiling (1)
- molecular diagnostic techniques (1)
- molecular subtyping (1)
- molecular typing (1)
- multiplexed immunofluorescence (1)
- neoadjuvant chemoradiotherapy (1)
- nodular sclerosis (1)
- oral cavity cancer (1)
- p16 (1)
- predictive biomarker (1)
- stomach neoplasms (1)
- transcriptome (1)
The hallmark of classical Hodgkin lymphoma (cHL) is the presence of giant, mostly multinucleated Hodgkin-Reed-Sternberg (HRS) cells. Whereas it has recently been shown that giant HRS cells evolve from small Hodgkin cells by incomplete cytokinesis and re-fusion of tethered sister cells, it remains unsolved why this phenomenon particularly takes place in this lymphoma and what the differences between these cell types of variable sizes are. The aim of the present study was to characterize microdissected small and giant HRS cells by gene expression profiling and to assess differences of clonal growth behavior as well as susceptibility toward cytotoxic intervention between these different cell types to provide more insight into their distinct cellular potential. Applying stringent filter criteria, only two differentially expressed genes between small and giant HRS cells, SHFM1 and LDHB, were identified. With looser filter criteria, 13 genes were identified to be differentially overexpressed in small compared to giant HRS cells. These were mainly related to energy metabolism and protein synthesis, further suggesting that small Hodgkin cells resemble the proliferative compartment of cHL. SHFM1, which is known to be involved in the generation of giant cells, was downregulated in giant RS cells at the RNA level. However, reduced mRNA levels of SHFM1, LDHB and HSPA8 did not translate into decreased protein levels in giant HRS cells. In cell culture experiments it was observed that the fraction of small and big HRS cells was adjusted to the basic level several days after enrichment of these populations via cell sorting, indicating that small and big HRS cells can reconstitute the full spectrum of cells usually observed in the culture. However, assessment of clonal growth of HRS cells indicated a significantly reduced potential of big HRS cells to form single cell colonies. Taken together, our findings pinpoint to strong similarities but also some differences between small and big HRS cells.