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Core Facilities (CF) for advanced light microscopy (ALM) have become indispensable support units for research in the life sciences. Their organizational structure and technical characteristics are quite diverse, although the tasks they pursue and the services they offer are similar. Therefore, throughout Europe, scientists from ALM‐CFs are forming networks to promote interactions and discuss best practice models. Here, we present recommendations for ALM‐CF operations elaborated by the workgroups of the German network of ALM‐CFs, German Bio‐Imaging (GerBI). We address technical aspects of CF planning and instrument maintainance, give advice on the organization and management of an ALM‐CF, propose a scheme for the training of CF users, and provide an overview of current resources for image processing and analysis. Further, we elaborate on the new challenges and opportunities for professional development and careers created by CFs. While some information specifically refers to the German academic system, most of the content of this article is of general interest for CFs in the life sciences.
Mit Nietzsches Rede von der "Wahrheit als Weib" verabschiedet sich die fröhliche Wissenschaft von jeder idealistischen Philosophie, die dem Phantasma der durch einen männlichen Akt epistemologischer Gewalt entschleierbaren 'nuda veritas' aufsitzt. Wenn man Nietzsches Bezug auf die mythologische Baubo und deren Geste der Selbstentblößung nachverfolgt, wird Nietzsches Praxis als Überwindung des Willens zum Wissen deutlich erkennbar. Denn jene Geste der Baubo, die sich jeder ultimativen Deutung entzieht, bleibt dennoch äußerst wirkmächtig, und das nicht nur für Nietzsche selbst: Der Text schlägt vor, Georges Batailles Mobilisierung der Figur des 'Acéphale' in den 1930er Jahren als Variation der Baubo zu lesen - dies auch, jedoch nicht nur aufgrund ikonografischer Affinitäten. Gleiches gilt für Jacques Lacans theatralische Inszenierung von Gustave Courbets Gemälde "L'origine du monde", einer wichtigen Quelle für dessen "Seminar III: Die Psychosen". Diese Filiationen der Baubo eröffnen ein Denken der Unmöglichkeit eines Wissens vom eigenen Ursprung, das kastrierend-bedrohlich, in seiner Rätselhaftigkeit fetischistisch reizend oder fröhlich-affirmativ besetzt werden kann. Ob die apotropäische Geste der Baubo als obszön, (unheil)schwanger, tröstend oder belustigend empfunden wird, an ihr stellt sich unvermeidlich die Frage nach der sexuellen Differenz und den Möglichkeiten der Symbolisierung des Weiblichen im männlichen Diskurs.
Classic Hodgkin lymphoma (cHL) is usually characterized by a low tumour cell content, derived from crippled germinal centre B cells. Rare cases have been described in which the tumour cells show clonal T-cell receptor rearrangements. From a clinicopathological perspective, it is unclear if these cases should be classified as cHL or anaplastic large T-cell lymphoma (ALCL). Since we recently observed differences in the motility of ALCL and cHL tumour cells, here, we aimed to obtain a better understanding of T-cell-derived cHL by investigating their global proteomic profiles and their motility. In a proteomics analysis, when only motility-associated proteins were regarded, T-cell-derived cHL cell lines showed the highest similarity to ALK− ALCL cell lines. In contrast, T-cell-derived cHL cell lines presented a very low overall motility, similar to that observed in conventional cHL. Whereas all ALCL cell lines, as well as T-cell-derived cHL, predominantly presented an amoeboid migration pattern with uropod at the rear, conventional cHL never presented with uropods. The migration of ALCL cell lines was strongly impaired upon application of different inhibitors. This effect was less pronounced in cHL cell lines and almost invisible in T-cell-derived cHL. In summary, our cell line-derived data suggest that based on proteomics and migration behaviour, T-cell-derived cHL is a neoplasm that shares features with both cHL and ALCL and is not an ALCL with low tumour cell content. Complementary clinical studies on this lymphoma are warranted.
Anaplastic large cell lymphoma (ALCL) and classical Hodgkin lymphoma (cHL) are lymphomas that contain CD30-expressing tumor cells and have numerous pathological similarities. Whereas ALCL is usually diagnosed at an advanced stage, cHL more frequently presents with localized disease. The aim of the present study was to elucidate the mechanisms underlying the different clinical presentation of ALCL and cHL. Chemokine and chemokine receptor expression were similar in primary ALCL and cHL cases apart from the known overexpression of the chemokines CCL17 and CCL22 in the Hodgkin and Reed-Sternberg (HRS) cells of cHL. Consistent with the overexpression of these chemokines, primary cHL cases encountered a significantly denser T cell microenvironment than ALCL. Additionally to differences in the interaction with their microenvironment, cHL cell lines presented a lower and less efficient intrinsic cell motility than ALCL cell lines, as assessed by time-lapse microscopy in a collagen gel and transwell migration assays. We thus propose that the combination of impaired basal cell motility and differences in the interaction with the microenvironment hamper the dissemination of HRS cells in cHL when compared with the tumor cells of ALCL.