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Bank regulators have the discretion to discipline banks by executing enforcement actions to ensure that banks correct deficiencies regarding safe and sound banking principles. We
highlight the trade-offs regarding the execution of enforcement actions for financial stability. Following this we provide an overview of the differences in the legal framework governing supervisors’ execution of enforcement actions in the Banking Union and the United States. After discussing work on the effect of enforcement action on bank behaviour and the real economy, we present data on the evolution of enforcement actions
and monetary penalties by U.S. regulators. We conclude by noting the importance of supervisors to levy efficient monetary penalties and stressing that a division of competences among different regulators should not lead to a loss of efficiency regarding
the execution of enforcement actions.
The centerpiece of all neuronal processes is the synaptic transmission. It consists of a complex series of events. Two key elements are the binding of synaptic vesicles (SV) to the presynaptic membrane and the subsequent fusion of the two membranes. SV are neurotransmitter-filled membranous spheres with many integral and peripheral proteins. The synaptic SNARE complex consists of three interacting proteins, which energize and regulate the fusion of the SV membrane with the presynaptic membrane. Both processes are closely orchestrated to ensure a specific release of neurotransmitter. Already many experiments have been performed, such as genetic screens and proteome analysis of SV, to determine the functions of the various proteins involved. Nevertheless, the functions of the identified proteins are still not fully elucidated. The aim of this thesis was initially applying a tandem affinity purification (TAP) of SV to identify unknown interaction partner of SV and to determine their role. This was supposed to be performed in the model organism Caenorhabditis elegans (C. elegans). The underlying mechanisms are conserved throughout the phylogentic tree and identified interaction partners will help to understand the processes in the mammalian brain. Although there is no neuron-rich tissue in C. elegans as in other model organisms, the diverse genetic methods allows a rapid creation of modified organisms and a prompt determination of the function of identified proteins. The integral SV protein synaptogyrin has been fused to a TAP-tag. The TAP-tag consists of a ProteinA, a TEV protease cleavage site and a calmodulin binding peptide (CBP). Both affinity purification steps are performed sequentially and allow a highly specific native purification of proteins and their interaction partners. Due to technical difficulties the purification strategy was modified several times during the course of this thesis and then finally abandoned for a more promising project, the SNARE complex purification. In conclusion, one of the reasons was the necessary lack of detergent.
The amended aim of this thesis has been the TAP of solubilized SNARE complex to identify unknown interaction partner and to determine their role. In order to increase the specificity of the purification, in terms of formed complexes, the two SNARE subunits, synaptobrevin (SNB-1 in C. elegans) and syntaxin (UNC-64 in C. elegans), were separately fused to the different affinity tags. As the modifications of the proteins could impair their function and lead to false interaction partners, their functionality was tested. For this purpose, the corresponding fusion constructs were expressed in strains with mutated snb¬1 and unc-64. Non-functional synaptic proteins display an altered course of paralysis in an aldicarb assay. The fusion proteins which were expressed in their respective mutant strains displayed a near to wild-type (WT) behavior in contrast to the naive mutant strains. Multiple TAP demonstrated SNB-1 signals in Western blot analysis and complex sets of proteins in the final elution step in a silver staining of SDS-PAGEs. These samples were sent with negative control (WT purification) for MS analysis to various cooperation partners. 119 proteins were identified which appeared only in data sets with SNARE proteins and not in WT samples. If proteins were detected in ≥ 2 SNARE positive MS analysis and had known neural functions or homologies to neuronal proteins in other species, they were selected for further analysis. These candidates were knocked down by RNAi and tested for synaptic function in a following aldicarb assay. The treatment with their specific RNAi resulted for mca-3 in a strong resistance, while frm-2, snap-29, ekl-6, klb-8, mdh-2, pfk-2, piki-1 and vamp-8 resulted in hypersensitivity. The most responsive genes frm-2, snap-29 and mca-3 were examined, whether they displayed a co-localization together with synaptobrevin in promoter fusion constructs or functional fusion constructs. In fluorescence microscopy images only MCA-3::YFP demonstrated neuronal expression.
In order to substantiate the synaptic nature and functionality of the MCA-3::YFP a swimming assay was performed. Here, fusion construct expressing strains, which contained mutated mca-3, were compared with untreated mutant strains and WT strains according to their behavior. In this swimming assay a partial restoration of WT behavior was shown in the MCA-3::YFP expressing mutant strains. Based on these data, we discovered with MCA 3 a new interaction partner of the SNARE complex. MCA-3 is a plasma membrane Ca2+-ATPase and was initially seen only in their role in the endocytosis. Its new putative role is the reduction of Ca2+ concentration at the bound SNARE complex. Since an interaction of syntaxin with Ca2+ channels has been demonstrated, it would be comprehensible to reduce the local concentration of Ca2+ to a minimum by tethering Ca2+ transporters to the SNARE complex.
The estimation of water balance components as well as water-related indicators on the land surface by means of global hydrological models have evolved in recent decades. Results of such models are frequently used in global- and continental-scale assessments of the current and future state of the terrestrial water cycle and provide a valuable data basis, e.g., for the Intergovernmental Panel on Climate Change. The Water – Global Assessment and Prognosis (WaterGAP) model is one of the state-of-the-art models in that field and has been in development and application for around 20 years. The evaluation, modification and application of WaterGAP is the subject of this thesis. In particular, the sensitivity of climate input data on radiation calculation and simulated water fluxes and storages is evaluated in the first part. Effects of model modification such as updated spatial input datasets, improved process representation or an alternative calibration scheme are the focus of the second part. Finally, three applications of WaterGAP give insight into the capabilities of that model, namely an estimate of global and continental water balance components, an assessment of groundwater depletion and the impact of climate change on river flow regimes. Model experiments, which are described in six journal papers as well as the appendices, were used as the basis for answering the total of 13 research questions. One of the major foci was to quantify the sensitivity of simulated water fluxes and storages to alternative climate input data. It was found that the handling of precipitation undercatch leads to the greatest difference in water balance components, especially in those areas where WaterGAP is not calibrated due to a lack of river discharge observations. The modifications of WaterGAP in the last few decades has led in general to an improved simulation of monthly river discharge, but process representation in semi-arid and arid regions still requires improvements. With the most current model version, WaterGAP 2.2b, and for the time period 1971–2000, river discharge to the oceans and inland sinks is estimated to be 40 000 km3 yr-1, whereas actual evapotranspiration is simulated as 70 500 km3 yr-1. Future research needs for WaterGAP in particular but also for the global hydrological model community in general are defined, promoting a community-driven effort for a robust assessment of the continental water cycle.
In this paper we propose a way forward towards increased financial resilience in times of growing disagreement concerning open borders, free trade and global regulatory standards. In light of these concerns, financial resilience remains a highly valued policy objective. We wish to contribute by suggesting an agenda of concrete, do-able steps supporting an enhanced level of resilience, combined with a deeper understanding of its relevance in the public domain.
First, remove inconsistencies across regulatory rules and territorial regimes, and ensure their credibility concerning implementation. Second, discourage the use of financial regulatory standards as means of international competition. Third, give more weight to pedagogically explaining the established regulatory standards in public, to strengthen their societal backing.