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  • Adams, Stefan (1)
  • Bojunga, Jörg (1)
  • Kurek, Ralf (1)
  • Kusterer, Klaus (1)
  • Lindhorst, Elmar (1)
  • Renneberg, Heiner (1)
  • Röddiger, Sandra Johanna (1)
  • Schumm-Draeger, Petra-Maria (1)
  • Stanisch, Michael (1)
  • Usadel, Klaus-Henning (1)
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  • 2000 (1)

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Keywords

  • molecular diagnostics (1)
  • polymerase chain reaction (1)
  • reverse transcription (1)
  • thyroglobulin mRNA (1)
  • thyroid cancer (1)

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  • Medizin (1)

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Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR (2000)
Bojunga, Jörg ; Röddiger, Sandra Johanna ; Stanisch, Michael ; Kusterer, Klaus ; Kurek, Ralf ; Renneberg, Heiner ; Adams, Stefan ; Lindhorst, Elmar ; Usadel, Klaus-Henning ; Schumm-Draeger, Petra-Maria
The sensitive detection of circulating tumour cells in patients with differentiated thyroid cancer may precede the detection of relapse by other diagnostic studies – such as serum thyroglobulin – and thus may have important therapeutic and prognostic implications. We performed reverse transcription-polymerase chain reaction (RT-PCR) on blood samples from patients diagnosed with thyroid disease using two different RT-PCR sensitivities. Additionally, tissue specificity of TG mRNA-expression was determined using RNA extracts from 27 different human tissues. The lower limit of detection was 50–100 TG mRNA producing cells/ml blood using a ‘normal’ RT-PCR sensitivity and 10–20 cells/ml blood using a ‘high’ sensitivity. With the normal sensitivity TG mRNA was detected in 9/13 patients with thyroid cancer and metastasis, 63/137 patients with a history of thyroid cancer and no metastasis, 21/85 with non-malignant thyroid disease and 9/50 controls. With the high sensitivity TG mRNA was detected in 11/13 patients with thyroid cancer and metastasis, 111/137 patients with a history of thyroid cancer and no metastasis, 61/85 with non-malignant thyroid disease and 41/50 controls. Interestingly, using the normal RT-PCR sensitivity TG mRNA transcripts are specific for thyroid tissue and detectable in the peripheral blood of controls and patients with thyroid disease, which correlates with a diagnosis of metastasized thyroid cancer. However, with a high RT-PCR sensitivity, TG mRNA expression was found not to be specific for thyroid tissue and was not correlated with a diagnosis of thyroid cancer in patients. As a consequence, to date TG mRNA detected by RT-PCR in the peripheral blood cannot be recommended as a tumour marker superior to TG serum-level.
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