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Kontingenz / Zufall
(2019)
Bis zum Jahr 2003, als Peter Vogt, auf dessen Habilitationsschrift "Kontingenz und Zufall. Eine Ideen- und Begriffsgeschichte" Verena Wirtz im Folgenden eingeht, Teil des von Hans Joas geleiteten Forschungsprojekts "Kontingenz und Moderne" wurde und der Begriff von der Peripherie ins Zentrum interdisziplinärer Forschung rückte, handelte es sich um eine noch nicht begriffene Geschichte. Dabei war 'Kontingenz' als Mode- und Schlagwort der klassischen Moderne längst zu einem Grundbegriff der Postmoderne avanciert. Zunächst und primär Gegenstand der Philosophie, dann Leitbegriff der Soziologie, Ökonomie und Politikwissenschaft, hat sich im vergangenen Jahrzehnt auch die kontingenzscheue Geschichtswissenschaft des Begriffs und Sachverhalts des Unverfügbaren in der Geschichte angenommen.
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.
