Biologische Hochschulschriften (Goethe-Universität)
Refine
Year of publication
- 2007 (50) (remove)
Document Type
- Doctoral Thesis (48)
- Article (1)
- Diploma Thesis (1)
Has Fulltext
- yes (50)
Is part of the Bibliography
- no (50)
Keywords
- NMR-Spektroskopie (3)
- Gentherapie (2)
- Hitzestress (2)
- Immunologie (2)
- Molekularbiologie (2)
- Molekülstruktur (2)
- Proteinfaltung (2)
- gene therapy (2)
- 2-Hydroxylase (1)
- 2-hydroxylase (1)
- Actin-bindende Proteine (1)
- Ageing (1)
- Alkaloide (1)
- Allergie (1)
- Altern (1)
- Alzheimer's disease (1)
- Alzheimer-Krankheit (1)
- Aminosäurensequenz (1)
- Amyloid (1)
- Anabolismus (1)
- Apolipoprotein E (1)
- Aromatase (1)
- Arzneimitteldesign (1)
- Aufreinigung (1)
- Axoninitialsegment (1)
- B-Lymphozyt (1)
- BH3 mimetics (1)
- BH3-Mimetika (1)
- Biochemische Analyse (1)
- Biomechanik (1)
- Biosynthese (1)
- Biotechnologie (1)
- Biotechnologische Industrie (1)
- Blitzlicht (1)
- Blitzlicht-Photolyse (1)
- Blood-Brain Barrier (1)
- Blut-Hirn-Schranke (1)
- Breast Cancer (1)
- Brustkrebs (1)
- Carotinoide (1)
- Central Nervous System (1)
- Chalydomonas (1)
- Channelrhodopsin (1)
- Chinchilla (1)
- Chlamydomonas (1)
- Chronischer Schmerz (1)
- Chronobiologie (1)
- Chronobiology (1)
- Clock genes (1)
- Cochlea (1)
- Coenzym (1)
- Cofaktor (1)
- Copper (1)
- Cyclooxygenasen (1)
- Cytoplasmatische Vererbung (1)
- DR5 (1)
- Death-receptor (1)
- Decapping (1)
- Delaunay-Triangulierung (1)
- Dictyostelium discoideum (1)
- Docking (1)
- Dornapparat (1)
- Dreidimensionale NMR-Spektroskopie (1)
- Drug Design (1)
- Drug Targeting (1)
- Dynamik (1)
- Endocrine disrupter (1)
- Endokrin wirksamer Stoff (1)
- Endokrinologie (1)
- Endothelprogenitorzellen (1)
- Entzündung (1)
- Enzym (1)
- Enzymaktivität (1)
- Enzymologie (1)
- Excitation (1)
- Exzitation (1)
- Flavobacterium (1)
- Flavobacterium spec P99-3 (1)
- Follikuläre dendritische Zellen (1)
- Fusionsinhibitor (1)
- Gemeinschwämme (1)
- Gene expression (1)
- Genexpression (1)
- Genregulation (1)
- Gerinnungsfaktor VIII (1)
- Glioblastom (1)
- Glykoprotein GP 41 (1)
- Grün fluoreszierendes Protein (1)
- HIV (1)
- HIV infection (1)
- HIV-Infektion (1)
- HPLC (1)
- Hefe (1)
- Hefeartige Pilze (1)
- Heterologe Genexpression (1)
- Hinterhorn <Rückenmark> (1)
- Hippocampus (1)
- Hirntumor (1)
- Hitzeschock-Proteine (1)
- Hydrolasen (1)
- Hyperalgesie (1)
- Hörschädigung (1)
- IMAC (1)
- Immortalisierung (1)
- Immunkrankheit (1)
- Immunology (1)
- Immunsuppression (1)
- Inhibition (1)
- Inquisition post mortem (1)
- Interaktionsanalyse (1)
- Interferon (1)
- Intrazellulärer Transport (1)
- Kalziumspeicher (1)
- Kernpilze (1)
- Koaktivatoren (1)
- Krebs (Medizin) (1)
- Kristallzüchtung (1)
- Kryoelektronenmikroskopie (1)
- Kryokonservierung (1)
- Kupfer (1)
- Kupferstoffwechsel (1)
- Lipidmembran (1)
- Lymphknoten (1)
- Lysozym (1)
- M87o (1)
- Magnetische Kernresonanz (1)
- Makromolekulare Chemie (1)
- Makromolekül (1)
- Mehrdimensionale NMR-Spektroskopie (1)
- Melatonin (1)
- Membran (1)
- Membranfusion (1)
- Membranlipide (1)
- Membranproteine (1)
- Microbielle rhodopsine (1)
- Mitochondria (1)
- Mitochondriale DNS (1)
- Mitochondrium (1)
- Mittelhirn (1)
- Monozyklase (1)
- Myxol (1)
- NCoA proteins (1)
- NCoA-Proteine (1)
- NMR spectroscopy (1)
- NMR-Spectroscopy (1)
- NMR-spectroscopy (1)
- Nanoparticle (1)
- Nanopartikel (1)
- Naturstoff (1)
- Neuronale Plastizität (1)
- Nichtsteroidales Antiphlogistikum (1)
- ORF1 (1)
- Offener Leserahmen (1)
- Onkologie (1)
- Organogenese (1)
- P-bodies (1)
- PGE2 (1)
- Parallelverarbeitung (1)
- Pestizidbelastung (1)
- Pflanzenstress (1)
- Pichia ciferrii (1)
- Pichia pastoris (1)
- Pimephales promelas (1)
- Plant stress (1)
- Polymere (1)
- Processing bodies (1)
- Promotor <Genetik> (1)
- Prostaglandine (1)
- Prostgandin E Synthasen (1)
- Proteasom (1)
- Proteasomeninhibitoren (1)
- Protein-Protein-Wechselwirkung (1)
- Proteine (1)
- Proteinsekretion (1)
- Proteintransport (1)
- RNA (1)
- RNA-folding (1)
- RNS (1)
- Reaktionskinetik (1)
- Retrotransposition (1)
- Rhodopsin (1)
- Rückenmark (1)
- Schleimpilze (1)
- Schmerz (1)
- SecYEG (1)
- Sensorrhodopsin (1)
- Sphingolipide (1)
- Sphingosin (1)
- Spine (1)
- Stress (1)
- Strukturbiologie (1)
- T-Lymphozyt (1)
- TRAIL (1)
- Targeted drug delivery (1)
- Tau-Protein (1)
- Telomerase (1)
- Testosteronreductase <Testosteron-5-alpha-Reductase> (1)
- Tierphysiologie (1)
- Todesrezeptor (1)
- Transcription (1)
- Transkription (1)
- Transplantation (1)
- Transponierbares Element (1)
- Uhrengene (1)
- VSV (1)
- Viral Infection (1)
- Virusinfektion (1)
- Voronoi-Diagramm (1)
- YidC (1)
- Zellskelett (1)
- Zentralnervensystem (1)
- Zirbeldrüse (1)
- actin (1)
- aggregopathy (1)
- auditory Midbrain (1)
- bacterial two hybrid system (1)
- bakterielles Two Hybrid System (1)
- biomechanics (1)
- calcium store (1)
- carotenoid biosynthesis (1)
- cisternal organelle (1)
- coactivators (1)
- coagulation factor VIII (1)
- cyclooxygenase (1)
- diurnal (1)
- dynamics (1)
- enzyme assay (1)
- enzyme inhibitor (1)
- follicular dendritic cells (1)
- fusion inhibitor (1)
- genetical engineering (1)
- gp41 (1)
- hearing loss (1)
- hen egg white lysozyme (1)
- hyperalgesia (1)
- immortalization (1)
- intracellular transport (1)
- lentiviraler Vektor (1)
- mPGES-1 (1)
- mRNA-Abbau (1)
- mRNA-Speicherung (1)
- membrane fusion (1)
- molecular biology (1)
- molecular structure (1)
- monocyclase (1)
- multidimensional nmr spectroscopy (1)
- neuronal plasticity (1)
- nicht-native Proteine (1)
- nociception (1)
- non-native proteins (1)
- organotin compound (1)
- pesticide (1)
- promoter (1)
- prostaglandin synthases (1)
- proteasomeinhibitors (1)
- protein folding (1)
- rationale Stammentwicklung (1)
- retrotransposition (1)
- sphingolipids (1)
- sphingosine (1)
- spinal cord (1)
- spine apparatus (1)
- tau protein (1)
- yeast (1)
- zisternale Organelle (1)
Institute
- Biowissenschaften (27)
- Biochemie und Chemie (18)
- Pharmazie (4)
- Frankfurt Institute for Advanced Studies (FIAS) (1)
- Georg-Speyer-Haus (1)
- Medizin (1)
Rhythmic changes in environmental lighting conditions have ever been the most reliable environmental cue for life on earth. Nature has therefore selected a genetically encrypted endogenous clock very early in evolution, as it provided cells and subsequently organisms with the ability to anticipate persevering periods of light and darkness. Rhythm generation within the mammalian circadian system is achieved by clock genes and their protein products. The mammalian endogenous master clock, which synchronizes the body to environmental time, is located in the suprachiasmatic nucleus (SCN) of the hypothalamus. As an integral part of the time-coding system, the pineal gland serves the need to tune the body to the temporal environment by the rhythmic nocturnal synthesis and immediate release of the hormone melatonin. In contrast to the transcriptional regulation of melatonin synthesis in rodents, a post-translational shaping is indicated in the human pineal gland. Another important mediator of circadian time and seasonality to the body is the pituitary gland. The aim of this work was to elucidate regulation of melatonin synthesis in the human pineal gland. Furthermore, presence and regulation of clock genes in the human pineal and pituitary gland, and in the SCN were analyzed. Therefore, human tissue, taken from regular autopsies, was analyzed simultaneously for different parameters involved in melatonin biosynthesis and circadian rhythm generation. Presented data demonstrate that post-mortem brain tissue can be used to detect the remnant profile of pre-mortem adaptive changes in neuronal activity. In particular, our results give strong experimental support for the idea that transcriptional mechanisms are not dominant for the generation of rhythmic melatonin synthesis in the human pineal gland. Together with data obtained for clock genes and their protein products in the pituitary, data presented here offer 1) a new working hypothesis for post-translational regulation of melatonin biosynthesis in the human pineal gland, and 2) a novel twist in the molecular competence of clock gene proteins, achieved by nucleo-cytoplasmic shuttling in neuronal and neuroendocrine human tissue. Furthermore, in this study, oscillations in abundance of clock gene proteins were demonstrated for the first time in the human SCN.