Function of p21 (Cip1/Waf1/CDKN1A) in migration and invasion of cancer and trophoblastic cells

Tumor progression and pregnancy have several features in common. Tumor cells and placental trophoblasts share many signaling pathways involved in migration and invasion. Preeclampsia, associated with impaired differentia
Tumor progression and pregnancy have several features in common. Tumor cells and placental trophoblasts share many signaling pathways involved in migration and invasion. Preeclampsia, associated with impaired differentiation and migration of trophoblastic cells, is an unpredictable and unpreventable disease leading to maternal and perinatal mortality and morbidity. Like in tumor cells, most pathways, in which p21 is involved, are deregulated in trophoblasts of preeclamptic placentas. The aim of the present study was to enlighten p21’s role in tumorigenic choriocarcinoma and trophoblastic cell lines. We show that knockdown of p21 induces defects in chromosome movement during mitosis, though hardly affecting proliferation and cell cycle distribution. Moreover, suppression of p21 compromises the migration and invasion capability of various trophoblastic and cancer cell lines mediated by, at least partially, a reduction of the extracellular signal-regulated kinase 3, identified using transcriptome-wide profiling, real-time PCR, and Western blot. Further analyses show that downregulation of p21 is associated with reduced matrix metalloproteinase 2 and tissue inhibitor of metalloproteinases 2. This work evinces that p21 is involved in chromosome movement during mitosis as well as in the motility and invasion capacity of trophoblastic and cancer cell lines.
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Metadaten
Author:Nina-Naomi Kreis, Alexandra Friemel, Andreas Ritter, Susanne Roth, Udo Rolle, Frank Louwen, Juping Yuan
URN:urn:nbn:de:hebis:30:3-512646
DOI:http://dx.doi.org/10.3390/cancers11070989
ISSN:2072-6694
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=31311187
Parent Title (English):Cancers
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/07/15
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/09/30
Tag:CDKN1A; ERK3/MAPK6; MMP2; TIMP2; invasion; p21; trophoblastic cell lines
Volume:11
Issue:7, Art. 989
Pagenumber:17
First Page:1
Last Page:17
Note:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
HeBIS PPN:454003234
Institutes:Medizin
Dewey Decimal Classification:610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Medizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0

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