- Treffer 1 von 1
Role of sorting nexin PaATG24 in autophagy, aging and development of Podospora anserina
- Sorting nexins are a conserved protein family involved in vesicle transport, membrane trafficking and protein sorting. The sorting nexin ATG24/SNX4 has been demonstrated to be involved in different autophagy pathways and in endosomal trafficking. However, its impact on cellular quality control and on aging and development is still elusive. Here we report studies analyzing the function of PaATG24 in the aging model Podospora anserina. Ablation of PaATG24 leads to a reduced growth rate, infertility, and to a pronounced lifespan reduction. These characteristics are accompanied by alterations of the morphology and size distribution of vacuoles and severe impairments in non-selective and selective autophagy of peroxisomes (pexophagy) and mitochondria (mitophagy). While general autophagy and pexophagy are almost completely blocked, a PaATG24-independent form of mitophagy is induced during aging. In the ΔPaAtg24 mutant a strong accumulation of peroxisomes occurs while mitochondrial abundance is only slightly increased. These mitochondria are partially affected in function. Most strikingly, although some PaATG24-independent mitophagy exists, it appears that this is not sufficient to remove dysfunctional mitochondria efficiently enough to prevent premature aging. Overall our data emphasize the key role of mitochondria in aging and of mitophagy in quality control to keep a population of “healthy” mitochondria during aging.
Verfasserangaben: | Vanessa HenkelGND, Lea SchürmannsORCiDGND, Miriam Brunner, Andrea HamannORCiD, Heinz D. OsiewaczORCiDGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-528846 |
DOI: | https://doi.org/10.1016/j.mad.2020.111211 |
ISSN: | 1872-6216 |
ISSN: | 0047-6374 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/32007577 |
Titel des übergeordneten Werkes (Englisch): | Mechanisms of ageing and development |
Verlag: | Elsevier Science |
Verlagsort: | Amsterdam [u. a.] |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Fertigstellung: | 2020 |
Datum der Erstveröffentlichung: | 30.01.2020 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 19.02.2020 |
Freies Schlagwort / Tag: | Aging; Autophagy; Mitophagy; Pexophagy; Podospora anserina; Sorting nexins |
Jahrgang: | 186 |
Ausgabe / Heft: | Art. 111211 |
Seitenzahl: | 11 |
Erste Seite: | 1 |
Letzte Seite: | 11 |
Bemerkung: | © 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). |
HeBIS-PPN: | 461423162 |
Institute: | Biowissenschaften / Biowissenschaften |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Lizenz (Deutsch): | Creative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0 |