ω-Azido fatty acids as probes to detect fatty acid biosynthesis, degradation, and modification
- FAs play a central role in the metabolism of almost all known cellular life forms. Although GC-MS is regarded as a standard method for FA analysis, other methods, such as HPLC/MS, are nowadays widespread but are rarely applied to FA analysis. Here we present azido-FAs as probes that can be used to study FA biosynthesis (elongation, desaturation) or degradation (β-oxidation) upon their uptake, activation, and metabolic conversion. These azido-FAs are readily accessible by chemical synthesis and their matization with high sensitivity by HPLC/MS, contributing a powerful tool to FA analysis, and hence, lipid analysis in general.
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| Author: | Alexander José PérezGND, Helge Björn BodeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-769695 |
| DOI: | https://doi.org/10.1194/jlr.M047969 |
| ISSN: | 0022-2275 |
| Parent Title (English): | Journal of Lipid Research |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Year of first Publication: | 2014 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/08 |
| Tag: | S-azidoacyl-N-acetylcysteamine; azido-fatty acids; click-chemistry; cyclooctyne; fatty acid desaturation; fatty acid metabolism; strained promoted cycloadditon; β-oxidation |
| Volume: | 55.2014 |
| Issue: | 9 |
| Page Number: | 5 |
| First Page: | 1897 |
| Last Page: | 1901 |
| Institutes: | Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
