Specification of SUMO1- and SUMO2-interacting motifs
- SUMO proteins are ubiquitin-related modifiers implicated in the regulation of gene transcription, cell cycle, DNA repair, and protein localization. The molecular mechanisms by which the sumoylation of target proteins regulates diverse cellular functions remain poorly understood. Here we report isolation and characterization of SUMO1- and SUMO2-binding motifs. Using yeast two-hybrid system, bioinformatics, and NMR spectroscopy we define a common SUMO-interacting motif (SIM) and map its binding surfaces on SUMO1 and SUMO2. This motif forms a beta-strand that could bind in parallel or antiparallel orientation to the beta2-strand of SUMO due to the environment of the hydrophobic core. A negative charge imposed by a stretch of neighboring acidic amino acids and/or phosphorylated serine residues determines its specificity in binding to distinct SUMO paralogues and can modulate the spatial orientation of SUMO-SIM interactions.
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| Author: | Christina-Maria HeckerGND, Matthias RabillerGND, Kaisa HaglundORCiD, Peter BayerORCiDGND, Ivan ĐikićORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-762612 |
| DOI: | https://doi.org/10.1074/jbc.M512757200 |
| ISSN: | 0021-9258 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/16524884 |
| Parent Title (English): | Journal of biological chemistry |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Year of first Publication: | 2006 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/04/22 |
| Volume: | 281.2006 |
| Issue: | 23, art. 16127 |
| Article Number: | 16127 |
| Page Number: | 11 |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
