- Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.
MetadatenVerfasserangaben: | Aneesha KohliORCiD, Lucie SauerheringORCiDGND, Sarah Katharina FehlingGND, Kevin KlannORCiDGND, Helmut GeigerGND, Stephan BeckerGND, Benjamin Florian KochORCiDGND, Patrick C. BaerORCiDGND, Thomas StreckerORCiDGND, Christian MünchORCiD |
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URN: | urn:nbn:de:hebis:30:3-838396 |
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DOI: | https://doi.org/10.26508/lsa.202201371 |
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ISSN: | 2575-1077 |
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Titel des übergeordneten Werkes (Englisch): | Life Science Alliance |
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Verlag: | EMBO Press |
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Verlagsort: | Heidelberg |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 02.02.2022 |
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Datum der Erstveröffentlichung: | 02.02.2022 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 23.04.2024 |
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Freies Schlagwort / Tag: | Cell Biology; Microbiology, Virology & Host Pathogen Interaction; Systems & Computational Biology |
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Jahrgang: | 5 |
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Ausgabe / Heft: | 5, art. e202201371 |
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Aufsatznummer: | e202201371 |
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Seitenzahl: | 19 |
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Erste Seite: | 1 |
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Letzte Seite: | 19 |
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Bemerkung: | The LC-MS/MS proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE (102) partner repository with the dataset identifier: PXD024398. |
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Institute: | Medizin |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
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