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Similipal Biosphere Reserve is a part of biotic province of Chhotanagpur Plateau. It has a representative ecosystem under Mahanadian biogeographic region. Its biodiversity is an assemblage representation of Western Ghats and North-East India. Regarding bryophytes this area was rather unexplored and the current investigation shows the occurrence of 33 taxa of bryophytes in this biosphere reserve and its neighboring areas. Each species has been enumerated with its ecological and distributional details.