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Erscheinungsjahr
- 1980 (1)
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- Englisch (1)
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Schlagworte
- 14C-Labeled Terpenoids (1)
- Geranylgeranyl Pyrophosphate (1)
- Kaurene (1)
- Squalene (1)
- cis`-Phytoene (1)
Institut
- Biochemie und Chemie (1) (entfernen)
Methods are described for an enzymatic preparation of 14C-labeled terpenoids. With a cell-free system of a white mutant of Phycomyces blakesleeanus (Mucoraceae) [14C]squalene and [14C- cis]phytoene can be synthesized from [2-14C]mevalonate. The application of norflurazon, a phenyl- pyridazinone herbicide, helps to increase the yield of squalene. Furthermore, the liquid endosperm of Echinocystis lobata (Cucurbitaceae) was used for the formation of either [14C(-)]kaurene from [14C]mevalonic acid or [14C-/ra/w]geranylgeranyl pyrophosphate in the presence of Amo 1618.
The hydrocarbons formed were purified by alumina-column chromatography and preparative thin-layer chromatography (TLC). Geranylgeranyl pyrophosphate was separated by DE-column chromatography followed by TLC.