Refine
Year of publication
- 2006 (1) (remove)
Document Type
- Article (1) (remove)
Language
- English (1)
Has Fulltext
- yes (1) (remove)
Is part of the Bibliography
- no (1) (remove)
Keywords
- PAGE (1)
- Phylogeographie (1)
- Pityogenes (1)
- SSCP (1)
- Scolytidae (1)
- mitochondrial DNA (1)
- mitochondriale DNA (1)
- phylogeography (1)
Pityogenes chalcographus is a widely distributed spruce pest in Eurasia (KNIZEK et al. 2005). In 70ies, E. Führer studied the intraspecific variation of this spruce bark beetle and detected race differentiation among European populations based on crossing experiments (FÜHRER 1977), morphological characters FÜHRER 1978) and allozyme electrophoresis (RITZENGRUBER 1990). In order to verify the hypothesis differentiation, we analysed diverse European P. chalcographus populations using the Cytochrome Oxidase gene (COI) of the mitochondrial DNA. The complete COI gene of 96 individuals was sequenced. In facilitate the screening of the European populations, we applied a PCR-SSCP method. This polyacrylamide electrophoresis technique offers a sensitive but inexpensive, rapid and convenient method for detecting polymorphisms, reducing the amount of samples that require sequencing (SUNNUCKS et al. 2000).