Refine
Year of publication
- 1975 (1)
Document Type
- Article (1)
Language
- English (1)
Has Fulltext
- yes (1) (remove)
Is part of the Bibliography
- no (1)
Keywords
- Methylmercury Containing Inactivator (1) (remove)
Institute
- Biochemie und Chemie (1) (remove)
Sulfhydryl Groups, Methylmercury Containing Inactivator, Coenzyme Analogue Nicotinamide-(S-methylmercury-thioinosine) dinucleotide was formed by reaction of nicotin amide-(6-thiopurine) dinucleotide with methylmercury chloride. The compound exhibits coenzyme properties in the test with LDH (Km=1.5 × 10-4 м , Vmax=12500) and LADH (Km=1.7 × 10-4 м, Vmax=27) and inactivates YADH and GAPDH. From incubations with LDH and LADH the mercury containing coenzyme could be regained by column chromatography. The compound seems to be qualified for the X-ray structure analysis of the coenzyme-enzyme complex for some dehyrogenases based on the proportion of the heavy metal.