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We present a mechanism for the separation of strangeness from antistrangeness in the deconfinement transition. For a net strangeness of zero in the total system, the population of s quarks is greatly enriched in the quark-gluon plasma, while the s¯ quarks drift into the hadronic phase. This separation could result in ‘‘strangelet’’ formation, i.e., metastable blobs of strange-quark matter, which could serve as a unique signature for quark-gluon plasma formation in heavy-ion collisions. PACS: 25.70.Np, 12.38.Mh