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Bryophytes (liverworts, mosses and hornworts) hold the basal most position among extant land plants. The present study deals with comparative analysis of available mitochondrial genomes of bryophytes using bioinformatics tools. These mitochondrial genomes belong to 2 liverworts, 1 moss and 2 hornworts. The analysis is based on multiple genome analysis through whole proteome comparison to detect conservation between mitochondrial genomes of bryophytes.