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Adamczyk, Leszek ; Agakishiev, Geydar ; Aggarwal, Madan Mohan ; Ahammed, Zubayer ; Alakhverdyants, A. V. ; Alekseev, Igor G. ; Alford, J. ; Anderson, Bryon D. ; Anson, Christopher Daniel ; Arkhipkin, Dmitry A. ; Averichev, Georgy S. ; Balewski, Jan ; Banerjee, A. ; Barnovska, Z. ; Beavis, D. R. ; Bellwied, Rene ; Betancourt, M. J. ; Betts, R. R. ; Bhasin, Anju ; Bhati, Ashok Kumar ; Bichsel, Hans ; Bielčík, Jaroslav ; Bielčíková, Jana ; Bland, Leslie C. ; Bordyuzhin, Igor G. ; Borowski, W. ; Bouchet, J. ; Brandin, Andrei V. ; Brovko, S. G. ; Bruna, Elena ; Bueltmann, S. ; Bunzarov, Ivan ; Burton, T. P. ; Butterworth, Joseph ; Cai, Xiang-Zhou ; Caines, Helen Louise ; Calderón de la Barca Sánchez, Manuel ; Cebra, Daniel A. ; Cendejas, R. ; Cervantes, M. C. ; Chaloupka, Petr ; Chattopadhyay, S. ; Chen, H. F. ; Chen, J. H. ; Chen, J. Y. ; Chen, L. ; Cheng, J. ; Cherney, Michael Gerard ; Chikanian, Alexei ; Christie, William ; Chung, P. ; Chwastowski, J. ; Codrington, M. J. M. ; Corliss, R. ; Cramer, John G. ; Crawford, Henry J. ; Cui, X. ; Davila Leyva, A. ; De Silva, L. C. ; Debbe, R. R. ; Dedovich, T. G. ; Deng, J. ; Souza, Rafael Derradi de ; Dhamija, S. ; Didenko, Lidia ; Ding, F. ; Djawotho, P. ; Dong, X. ; Drachenberg, James Lucus ; Draper, James E. ; Du, C. M. ; Dunkelberger, L. E. ; Dunlop, James C. ; Efimov, Leonid G. ; Elnimr, M. ; Engelage, Jon M. ; Eppley, Geary Wareham ; Eun, L. ; Evdokimov, Olga Y. ; Fatemi, Renee H. ; Fedorišin, Jan ; Fersch, R. G. ; Filip, Peter ; Finch, Evan ; Fisyak, Yuri V. ; Gagliardi, Carl A. ; Gangadharan, Dhevan Raja ; Geurts, Frank ; Gliske, S. ; Gorbunov, Y. N. ; Grebenyuk, O. G. ; Grosnick, David P. ; Gupta, S. ; Guryn, Włodzimierz ; Haag, B. ; Hajkova, O. ; Hamed, Ahmed M. ; Han, L-X. ; Harris, John W. ; Hays-Wehle, J. P. ; Heppelmann, S. ; Hirsch, Andrew S. ; Hoffmann, Gerald W. ; Hofman, D. J. ; Horvat, S. ; Huang, Bingchu ; Huang, Huan Zhong ; Huck, P. ; Humanic, Thomas J. ; Huo, L. ; Igo, George J. ; Jacobs, William W. ; Jena, Chitrasen ; Joseph, J. ; Judd, Eleanor G. ; Kabana, Sonia ; Kang, K. ; Kapitan, J. ; Kauder, Kolja ; Ke, Hongwei ; Keane, Declan ; Kechechyan, Armen ; Kesich, A. ; Kettler, D. ; Kikola, D. P. ; Kiryluk, Joanna ; Kisiel, Adam Ryszard ; Kizka, V. ; Klein, Spencer Robert ; Koetke, Donald D. ; Kollegger, Thorsten ; Konzer, J. ; Koralt, I. ; Koroleva, L. ; Korsch, W. ; Kotchenda, L. ; Kravtsov, Peter ; Krueger, Keith William ; Kumar, Lokesh ; Lamont, Matthew A. C. ; Landgraf, Jeffery M. ; LaPointe, S. ; Lauret, Jerome ; Lebedev, Alexei ; Lednicky, Richard ; Lee, J. H. ; Leight, W. ; LeVine, Micheal J. ; Li, C. ; Li, L. ; Li, W. ; Li, X. ; Li, X. ; Li, Y. ; Li, Z. M. ; Lima, L. M. ; Lisa, Michael A. ; Liu, F. ; Ljubicic, Tonko A. ; Llope, William Joseph ; Longacre, Ronald S. ; Lu, Y. ; Luo, X. ; Luszczak, A. ; Ma, G. L. ; Ma, Yu-Gang ; Mahapatra, Durga Prasad ; Majka, Richard Daniel ; Mall, O. I. ; Margetis, Spyridon ; Markert, Christina ; Masui, H. ; Matis, Howard S. ; McDonald, Daniel ; McShane, Tom S. ; Mioduszewski, Saskia ; Mitrovski, M. K. ; Mohammed, Y. ; Mohanty, Bedangadas ; Morozov, B. ; Munhoz, Marcelo Gameiro ; Mustafa, M. K. ; Naglis, M. ; Nandi, Basanta Kumar ; Nasim, Md. ; Nayak, Tapan Kumar ; Nogach, Larisa V. ; Odyniec, Grazyna Janina ; Ogawa, Akio ; Oh, K. ; Ohlson, Alice ; Okorokov, Vitaly A. ; Oldag, E. W. ; Oliveira, R. A. N. ; Olson, Douglas L. ; Pachr, Miloš ; Page, Brian S. ; Pal, Susanta Kumar ; Pan, Y. X. ; Pandit, Y. ; Panebratsev, Yuri ; Pawlak, Tomasz Jan ; Pawlik, Bogdan ; Pei, Hua ; Perkins, Chris ; Peryt, Wiktor Stanislaw ; Pile, P. ; Planinic, Mirko ; Pluta, Jan Marian ; Plyku, D. ; Poljak, Nikola ; Porter, Jeffrey Brent ; Poskanzer, Arthur M. ; Powell, C. B. ; Prindle, Duncan J. ; Pruneau, Claude A. ; Pruthi, Navneet Kumar ; Przybycien, Mariusz ; Pujahari, P. R. ; Putschke, Jörn H. ; Qiu, H. ; Raniwala, Rashmi ; Raniwala, Sudhir ; Ray, Robert L. ; Redwine, R. ; Reed, Rosi Jan ; Riley, C. K. ; Ritter, Hans Georg ; Roberts, Jabus B. ; Rogačevskij, Oleg V. ; Romero, Juan L. ; Ruan, L. ; Rusnak, Jan ; Sahoo, Nihar Ranjan ; Sakrejda, Iwona ; Salur, Sevil ; Sandweiss, Jack H. ; Sangaline, E. ; Sarkar, Amal ; Schambach, Joachim ; Scharenberg, Rolf Paul ; Schmah, A. M. ; Schmitz, Norbert ; Schuster, T. R. ; Seele, J. ; Seger, Janet Elizabeth ; Seyboth, Peter ; Shah, N. ; Shahaliev, Ehtiram ; Shao, M. ; Sharma, B. ; Sharma, M. ; Shi, Shusu ; Shou, Q. Y. ; Sichtermann, Ernst P. ; Singaraju, Rama Narayana ; Skoby, Michael J. ; Smirnov, Nikolai ; Solanki, D. ; Sorensen, Paul Richard ; deSouza, U. G. ; Spinka, Harold. M. ; Srivastava, B. ; Stanislaus, T. D. Shirvel ; Steadman, S. G. ; Stevens, J. R. ; Stock, Reinhard ; Strichanov, Michail Nikolaevič ; Stringfellow, Blair ; Suaide, Alexandre Alarcon do Passo ; Suarez, M. C. ; Šumbera, Michal ; Sun, X. M. ; Sun, Y. ; Sun, Z. ; Surrow, Bernd ; Svirida, Dmitry N. ; Symons, Timothy James MacNeil ; Toledo, Alejandro Szanto de ; Takahashi, Jun ; Tang, Aihong ; Tang, Zebo ; Tarini, L. H. ; Tarnowsky, Terence J. ; Thein, Dylan ; Thomas, James H. ; Tian, J. ; Timmins, Anthony Robert ; Tlusty, David ; Tokarev, Mikhail V. ; Trainor, Thomas A. ; Trentalange, Stephen ; Tribble, Robert E. ; Tribedy, Prithwish ; Trzeciak, Barbara Antonina ; Tsai, Oleg D. ; Turnau, J. ; Ullrich, Thomas ; Underwood, David G. ; Van Buren, Gene ; van Nieuwenhuizen, G. ; Vanfossen, J. A. ; Varma, Raghava ; Vasconcelos, G. M. S. ; Videbæk, Flemming ; Viyogi, Yogendra P. ; Vokal, Stanislav ; Voloshin, Sergei ; Vossen, A. ; Wada, M. ; Wang, F. ; Wang, G. ; Wang, H. ; Wang, J. S. ; Wang, Q. ; Wang, X. L. ; Wang, Y. ; Webb, Grant ; Webb, Jason C. ; Westfall, Gary D. ; Whitten, Charles A. ; Wieman, Howard ; Wissink, Scott W. ; Witt, Richard ; Witzke, W. ; Wu, Y. F. ; Xiao, Z. ; Xie, W. ; Xin, K. ; Xu, H. ; Xu, N. ; Xu, Qinghua ; Xu, W. ; Xu, Y. ; Xu, Z. ; Xue, L. ; Yang, Y. ; Yang, Y. ; Yepes, Pablo P. ; Yi, Y. ; Yip, K. ; Yoo, I-K. ; Zawisza, M. ; Zbroszczyk, Hanna Paulina ; Zhang, J. B. ; Zhang, S. ; Zhang, W. M. ; Zhang, X. P. ; Zhang, Y. ; Zhang, Z. P. ; Zhao, F. ; Zhao, J. ; Zhong, C. ; Zhu, X. ; Zhu, Y. H. ; Zoulkarneeva, Youlia
STAR's measurements of directed flow (v1) around midrapidity for π±, K±, K0S, p and p¯ in Au + Au collisions at $\sqrtsNN = 200$ GeV are presented. A negative v1(y) slope is observed for most of produced particles (π±, K±, K0S and p¯). The proton v1(y) slope is found to be much closer to zero compared to antiprotons. A sizable difference is seen between v1 of protons and antiprotons in 5-30% central collisions. The v1 excitation function is presented. Comparisons to model calculations (RQMD, UrQMD, AMPT, QGSM with parton recombination, and a hydrodynamics model with a tilted source) are made. Anti-flow alone cannot explain the centrality dependence of the difference between the v1(y) slopes of protons and antiprotons.
Zum virologischen Nachweis einer akuten Influenza und zur Überprüfung des Immunstatus steht eine Vielzahl von Untersuchungsmethoden zur Verfügung. Bei Verdacht auf eine Influenzavirusinfektion liefert der Rachenabstrich das geeignete Untersuchungsmaterial. Das tiefe Nasopharynxaspirat ist etwas sensitiver, Sputum etwas weniger ergiebig. Die RT-PCR ermöglicht in 1–2 h nach Materialeingang ein sensitives und spezifisches Ergebnis. Typen, Subtypen und Driftvarianten lassen sich durch geeignete Primersonden, die kommerziell zur Verfügung stehen, einwandfrei identifizieren. Demgegenüber ist die Zellkultur-gestützte Virusisolierung zeitaufwendiger und stärker abhängig von einer sachgerechten Materialgewinnung und –überbringung (Kühlkette). PCR und Virusanzüchtung ermöglichen die geno- bzw. phänotypische Testung auf Therapieresistenzen. Der Antigentest ist eine einfache (bed-side) Schnellmethode. Seine Spezifität ist gut, die Sensitivität limitiert; daher kann der Antigentest nicht zur individuellen Ausschlussdiagnose eingesetzt werden. Influenzavirusspezifische Antikörper erscheinen im Blut erst in der zweiten Krankheitswoche. Die Serodiagnostik erfolgt typenspezifisch mit Komplementbindungsreaktion (KBR), IFT und ELISA über eine signifikante Titerbewegung oder den Nachweis von IgA-Antikörpern. IgG-spezifische IFT und ELISA Methoden geben Auskunft über die Influenzavirus-typspezifische Durchseuchung. Die klinisch relevantere subtypen- und variantenspezifische Influenzavirusimmunität wird mit dem HHT oder NT gemessen.
Die Labordiagnose einer Infektionskrankheit beruht auf dem Nachweis des Infektionserregers oder der spezifischen Immunreaktion unter Berücksichtigung der klinischen Plausibilität. Biologische Testverfahren wie der Zellkulturversuch erbringen nur näherungsweise ein quantitatives Ergebnis und sind mit einer relativ großen Streuung behaftet. Das gilt auch für Antikörperassays, soweit sie über ein biologisches Testsignal abgelesen werden (CPE, Agglutination, Komplementverbrauch). Moderne serologische und molekularbiologische Untersuchungsmethoden der Virologie werden i. d. R. über ein physikochemisches Testssignal abgelesen und quantitativ ausgewertet. Dadurch gelingt die nationale und internationale Standardisierung, die sich in Ringversuchen gut überprüfen lässt. Aus biologischen Gründen ist meist eine log. Ergebnisberechnung angezeigt, was für „ signifikante “ Unterschiede in Verlaufsuntersuchungen zu berücksichtigen ist: Da sowohl Infektion als auch Immunreaktion dynamische Prozesse darstellen, können Normalwerte in der virologischen Labordiagnostik nur restriktiv definiert werden. Ihre Ergebnisse sind mehr oder minder individuell interpretationsbedürftig.
Wycisk, Agnes Isabella ; Lin, Jiacheng ; Loch, Sandra ; Hobohm, Kathleen ; Funke, Jessica ; Wieneke, Ralph ; Koch, Joachim ; Skach, William R. ; Mayerhofer, Peter Uli ; Tampé, Robert
Virus-infected cells are eliminated by cytotoxic T lymphocytes, which recognize viral epitopes displayed on major histocompatibility complex class I molecules at the cell surface. Herpesviruses have evolved sophisticated strategies to escape this immune surveillance. During the lytic phase of EBV infection, the viral factor BNLF2a interferes with antigen processing by preventing peptide loading of major histocompatibility complex class I molecules. Here we reveal details of the inhibition mechanism of this EBV protein. We demonstrate that BNLF2a acts as a tail-anchored protein, exploiting the mammalian Asna-1/WRB (Get3/Get1) machinery for posttranslational insertion into the endoplasmic reticulum membrane, where it subsequently blocks antigen translocation by the transporter associated with antigen processing (TAP). BNLF2a binds directly to the core TAP complex arresting the ATP-binding cassette transporter in a transport-incompetent conformation. The inhibition mechanism of EBV BNLF2a is distinct and mutually exclusive of other viral TAP inhibitors.
Holland, Sabrina Marion ; Coste, Ovidiu ; Zhang, Dong Dong ; Pierre, Sandra C. ; Geisslinger, Gerd ; Scholich, Klaus
The E3 ubiquitin ligase MYCBP2 negatively regulates neuronal growth, synaptogenesis, and synaptic strength. More recently it was shown that MYCBP2 is also involved in receptor and ion channel internalization. We found that mice with a MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Surprisingly, loss of MYCBP2 inhibited internalization of transient receptor potential vanilloid receptor 1 (TRPV1) and prevented desensitization of capsaicin-induced calcium increases. Lack of desensitization, TRPV internalization and prolonged hyperalgesia were reversed by inhibition of p38 MAPK. The effects were TRPV-specific, since neither mustard oil-induced desensitization nor behavioral responses to mechanical stimuli were affected. In summary, we show here for the first time that p38 MAPK activation can inhibit activity-induced ion channel internalization and that MYCBP2 regulates internalization of TRPV1 in peripheral sensory neurons as well as duration of thermal hyperalgesia through p38 MAPK.