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Due to an increasing awareness of the potential hazardousness of air pollutants, new laws, rules and guidelines have recently been implemented globally. In this respect, numerous studies have addressed traffic-related exposure to particulate matter using stationary technology so far. By contrast, only few studies used the advanced technology of mobile exposure analysis. The Mobile Air Quality Study (MAQS) addresses the issue of air pollutant exposure by combining advanced high-granularity spatial-temporal analysis with vehicle-mounted, person-mounted and roadside sensors. The MAQS-platform will be used by international collaborators in order 1) to assess air pollutant exposure in relation to road structure, 2) to assess air pollutant exposure in relation to traffic density, 3) to assess air pollutant exposure in relation to weather conditions, 4) to compare exposure within vehicles between front and back seat (children) positions, and 5) to evaluate "traffic zone"- exposure in relation to non-"traffic zone"-exposure. Primarily, the MAQS-platform will focus on particulate matter. With the establishment of advanced mobile analysis tools, it is planed to extend the analysis to other pollutants including including NO2, SO2, nanoparticles, and ozone.
Background: Hemorrhagic shock/resuscitation is associated with aberrant neutrophil activation and organ failure. This experimental porcine study was done to evaluate the effects of Fas-directed extracorporeal immune therapy with a leukocyte inhibition module (LIM) on hemodynamics, neutrophil tissue infiltration, and tissue damage after hemorrhagic shock/resuscitation. Methods: In a prospective controlled double-armed animal trial 24 Munich Mini Pigs (30.3 +/- 3.3 kg) were rapidly haemorrhaged to reach a mean arterial pressure (MAP) of 35 +/- 5 mmHg, maintained hypotensive for 45 minutes, and then were resuscitated with Ringer's solution to baseline MAP. With beginning of resuscitation 12 pigs underwent extracorporeal immune therapy for 3 hours (LIM group) and 12 pigs were resuscitated according to standard medical care (SMC). Haemodynamics, haematologic, metabolic, and organ specific damage parameters were monitored. Neutrophil infiltration was analyzed histologically after 48 and 72 hours. Lipid peroxidation, and apoptosis were specifically determined in lung, bowel, and liver. Results: In the LIM group, neutrophil counts were reduced versus SMC during extracorporeal immune therapy. After 72 hours, the haemodynamic parameters MAP and cardiac output (CO) were significantly better in the LIM group. Histological analyses showed reduction of shock-related neutrophil tissue infiltration in the LIM group, especially in the lungs. Lower amounts of apoptotic cells and lipid peroxidation were found in organs after LIM treatment. Conclusions: Transient Fas-directed extracorporeal immune therapy may protect from posthemorrhagic neutrophil tissue infiltration and tissue damage.
Objective: To summarise the benefits and harms of treatments for women with gestational diabetes mellitus. Design: Systematic review and meta-analysis of randomised controlled trials. Data sources: Embase, Medline, AMED, BIOSIS, CCMed, CDMS, CDSR, CENTRAL, CINAHL, DARE, HTA, NHS EED, Heclinet, SciSearch, several publishers’ databases, and reference lists of relevant secondary literature up to October 2009. Review methods: Included studies were randomised controlled trials of specific treatment for gestational diabetes compared with usual care or "intensified" compared with "less intensified" specific treatment. Results: Five randomised controlled trials matched the inclusion criteria for specific versus usual treatment. All studies used a two step approach with a 50 g glucose challenge test or screening for risk factors, or both, and a subsequent 75 g or 100 g oral glucose tolerance test. Meta-analyses did not show significant differences for most single end points judged to be of direct clinical importance. In women specifically treated for gestational diabetes, shoulder dystocia was significantly less common (odds ratio 0.40, 95% confidence interval 0.21 to 0.75), and one randomised controlled trial reported a significant reduction of pre-eclampsia (2.5 v 5.5%, P=0.02). For the surrogate end point of large for gestational age infants, the odds ratio was 0.48 (0.38 to 0.62). In the 13 randomised controlled trials of different intensities of specific treatments, meta-analysis showed a significant reduction of shoulder dystocia in women with more intensive treatment (0.31, 0.14 to 0.70). Conclusions: Treatment for gestational diabetes, consisting of treatment to lower blood glucose concentration alone or with special obstetric care, seems to lower the risk for some perinatal complications. Decisions regarding treatment should take into account that the evidence of benefit is derived from trials for which women were selected with a two step strategy (glucose challenge test/screening for risk factors and oral glucose tolerance test).
Feral cats (Felis catus), introduced into Australia with European settlers in the 19th century, colonized the entire Australian continent in less than 100 years, including the Australian arid zone which covers more than 70% of the continent. Feral cats are responsible for the decline and extinction of a number of native species and the failure of a number of reintroduction attempts, especially in the arid zone. Many ecological studies on feral cats have been conducted on home range size and movement patterns in different environments, abundance and diet, with the aim of gaining a better understanding about their successful invasion of the Australian continent. There are no physiological studies on the feral cat to date. However, there is evidence that there is a strong interrelation between physiology and abiotic factors such as climate. Thus, distribution, habitat, and dispersal of species can not fully be understood without background knowledge of physiology. This PhD aims to contribute to a better understanding of three physiological parameters: metabolism, body mass and body temperature patterns. These parameters may possibly identify physiological adaptation to different climate zones, seasonal conditions and island isolation.
Until now, the NW Indian Ocean was sparsely covered with coral proxy records, and records from the Maldives Archipelago do not exist. The first such coral proxy record from the central Maldives is presented in this study. It originates from a massive Porites lutea (Quoy and Gaimard, 1833) colony that was sampled March 2007 in the lagoon of Rasdhoo Atoll (4°N/ 73°W), which is located in the central Maldives. The record spans a period of 90 yrs and reaches back to 1917 AD with monthly to bimonthly resolution. This study investigates temporal variations of the skeletal stable oxygen (delta18O) and carbon (delta13C) isotopes, the strontium-to-calcium (Sr/Ca), and the annual extension-rates, and their relationship to historical climate variations 1917-2007. Annual extension-rates show an increase over the 20th century, and are correlated with instrumental sea surface temperatures (SST). The interannual variation of the extension-rates within 2.5-4 years is driven by the El Niño-Southern Oscillation (ENSO). The amount of skeletal extension during the summer months is triggered by variations in the strength of the SW monsoon. Interannual and decadal variability in monsoon current activity (18-19 yrs) and rainfall over India are an expression of the summer monsoon strength. This is the reason why a statistical link between coral extension-rates and precipitation over India can be established. This implies that annual extension-rates in corals can be used as a new proxy for Indian monsoon variability on decadal resolution. The delta18O record exhibits the 20th century warming trend that is influenced by the effect of monsoon-induced cooling. delta18O also reveals interannual ENSO triggered variability, which is due to ENSO-forced variations in SST and sea surface salinity (SSS). A decadal variation at 12-14 yrs cannot be linked to SST variations in the NW Indian Ocean, but with decadal variations of SSS. They could be caused by ENSO- forced variations of the monsoon currents during the mature phase of ENSO teleconnections in the Indian Ocean in boreal winter. The Sr/Ca record does not indicate a significant warming, in spite of the observed SST rise at the sampling site. Changes in seawater Sr/Ca cannot be excluded. Nevertheless, interannual ENSO forcing is still evident. Evidence for the Pacific Decadal Oscillation (PDO) is found during 1917-1955. Afterwards, the Sr/Ca data indicate the disappearance of PDO forcing. By the combination of Sr/Ca and delta18O it is possible to detect ~80% of historical El Niño and La Niña events at the sample site. This study confirms the notion that interannual to multi-decadal climate fluctuations in the Pacific play a crucial role for climate variability in the Indian Ocean.
In this thesis, we study the properties of excitations in the systems of interacting fermions. These excitations can be bosonic such as collective modes which we handle in the first part of this thesis or fermionic like quasi particles and quasi holes. One of the important points, to investigate the excitations is their damping which corresponds to their life-time in the system. This thesis consists of two parts, where in both parts, we use the field-theoretical methods to examine the problem.
Background: Previously, we showed that glioma pathogenesis related protein (GliPR) is induced in CEM T cells upon HIV-1 infection in vitro. To examine whether GliPR plays a role as HIV dependency factor (HDF), we tested the effect of GliPR suppression by siRNA on HIV-1 replication. Results: Induction of GliPR expression by HIV-1 was confirmed in P4-CCR5 cells. When GliPR was suppressed by siRNA, HIV-1 replication was significantly reduced as measured by HIV-1 transcript levels, HIV-1 p24 protein levels, and HIV-1 LTR-driven reporter gene expression, suggesting that GliPR is a cellular co-factor of HIV-1. Microarray analysis of uninfected HeLa cells following knockdown of GliPR revealed, among a multitude of gene expression alterations, a down-regulation of syndecan-1, syndecan-2, protein kinase C alpha (PRKCA), the catalytic subunit beta of cAMP-dependent protein kinase (PRKACB), nuclear receptor co-activator 3 (NCOA3), and cell surface protein CD59 (protectin), all genes having relevance for HIV-1 pathology. Conclusions: The up-regulation of GliPR by HIV-1 and the early significant inhibition of HIV-1 replication mediated by knockdown of GliPR reveal GliPR as an important HIV-1 dependency factor (HDF), which may be exploited for HIV-1 inhibition.
In der Doktorarbeit wurde ein Verfahren zur Ermittlung der Schwerpunkthöhe eines Fahrzeugs aus den Messwerten von Sensoren, die serienmäßig in vielen geländegängigen Fahrzeugen verbaut sind, entwickelt. Dieses Verfahren benötigt nur die Signale von Sensoren des elektronischen Stabilitätssystems (ESP) und eines Fahrwerks mit Luftfeder. Um die Höhe des Schwerpunkts zu bestimmen, wurde ein Modell entworfen, das die Drehbewegung des Fahrzeugs um seine Längsachse beschreibt. Eine der unbekannten Größen in diesem Modell ist das Produkt m_g\Deltah, wobei mit m_g die gefederte Masse des Fahrzeugs und mit Deltah der Abstand zwischen dem Schwerpunkt und der Wankachse des Fahrzeugs bezeichnet wird. Die Höhe des Schwerpunkts wird berechnet, indem zu diesem Abstand der als bekannt vorausgesetzte Abstand der Wankachse von der Straße addiert wird. Es wurden drei Varianten des Modells betrachtet. Die eine Modellvariante (stationäres Modell) beschreibt das Fahrzeugverhalten nur in solchen Fahrsituationen exakt, in denen die Wankgeschwindigkeit und die Wankbeschleunigung vernachlässigbar klein sind. In dieser Modellvariante wurden die Federkräfte mit einem detaillierten Modell der Luftfeder berechnet. Eine Eingangsgröße dieses Modells ist der Druck in den Gummibälgen der Luftfeder. Um diesen Druck zu ermitteln, wurde ein Algorithmus auf dem Steuergerät des Luftfedersystems implementiert. Um die Genauigkeit des Luftfedermodells zu testen und um die Abmessungen bestimmter Bauteile der Luftfeder zu ermitteln, wurden Messungen am Federungsprüfstand durchgeführt und eine Methode entwickelt, wie aus diesen Messungen die gesuchten Größen berechnet werden können. Bei den zwei übrigen Modellvarianten (dynamisches Modell) gelten die Einschränkung für die Fahrsituationen nicht. Die einzelnen Varianten des dynamischen Modells unterscheiden sich darin, dass das eine Mal die Feder- und Dämpferkonstanten als bekannt vorausgesetzt und das andere Mal aus den Sensorsignalen geschätzt werden. Passend zu jeder Modellvariante wurde ein Verfahren gewählt, mit dem Schätzwerte für das Produkt m_g\Deltah berechnet wurden. Des Weiteren wurde auch eine Methode entwickelt, mit der die Masse mg geschätzt wurde, ohne zuvor ein Wert für das Produkt m_g\Deltah zu ermitteln. Die Schätzwerte wurden unter Verwendung von Daten ermittelt, die bei einer Simulation und bei Messfahrten gewonnen worden sind. Das Ergebnis des Vergleiches der betrachteten Modellvarianten ist, dass die eine Variante des dynamischen Modells zum Teil falsche Werte für m_g\Deltah liefert, weil die Modellgleichungen ein nicht beobachtbares System bilden. Die andere Variante dieses Modells liefert nicht bei jeder Beladung exakte Werte, was vor allem daran liegt, dass in den Modellgleichungen dieses Modells ein konstanter Wert für die Federsteifigkeit angenommen wird. Bei Fahrzeugen mit Luftfeder ändert sich jedoch dieser Wert in Abhängigkeit von der Fahrzeugmasse. Die Werte von m_g\Deltah und mg können am genauesten mit dem stationären Modell ermittelt werden. Des Weiteren wurden Methoden entwickelt, die die Genauigkeit der durch den Schätzalgorithmus ermittelten Werte verbessern. So wurde zusätzlich zu dem Produkt m_g\Deltah und der Masse mg auch die Verteilung des Gewichtes auf die Vorder- und Hinterachse betrachtet. Es wurde ermittelt, welche Zusammenhänge zwischen dieser Verteilung und dem Produkt m_g\Deltah sowie zwischen dieser Verteilung und der Masse des Fahrzeugs bestehen. So konnte der Fehler in den Schätzwerten dieser Größen minimiert werden. Außerdem wurde auch der Zusammenhang zwischen dem Produkt m_g\Deltah und der Masse des Fahrzeugs ermittelt. Damit konnten die Schätzwerte dieser Größen genauer bestimmt werden. Aus den so gewonnenen Werten kann die Schwerpunkthöhe von einem Mercedes ML auf etwa 8cm genau berechnet werden. Diese Genauigkeit reicht aus, um das elektronische Stabilitätsprogramm auf die aktuelle Beladung des Fahrzeugs abzustimmen und damit einen Gewinn an Agilität für dieses Fahrzeug zu realisieren.