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The chenopodium aphid, Hayhurstia atriplicis (Linnaeus, 1761) (Hemiptera: Aphididae), is reported for the first time in Colombia. The aphid was found on the aerial parts of the quinoa plant, Chenopodium quinoa Willd. (Amaranthaceae), especially on the leaves, in five municipalities in the department of Cauca, Colombia. Brief information on its natural enemies, distribution and biology is provided. This is the only aphid in Colombia known to cause malformations on the leaves of quinoa plants. In South America, this aphid has been found previously only in Ecuador, where it was reported for the first time in 2023 based on specimens collected from 2019 to 2021. However, we report that H. atriplicis was likely introduced to Ecuador in the 2000s or earlier, based on a collection dated 2006 found in the United States National Museum of Natural History.
ZooBank registration. urn:lsid:zoobank.org:pub:F43613E0-2854-44DE-9654-74138909717D
First record of the beekeeping pest Aethina tumida Murray (Coleoptera: Nitidulidae) for Honduras
(2024)
Aethina tumida Murray (Coleoptera: Nitidulidae) (also known as the small hive beetle), an important pest in apiaries, is reported from Honduras for the first time, new country record. The precise distribution in the country is unknown, and no damage related to it has been observed. Photographs are provided to aid in its identification. Differentiation of A. tumida from Aethina villosa Reitter and Aethina quadrata Sharp, which also occur in Honduras, is provided.
ZooBank registration. urn:lsid:zoobank.org:pub:DAF12C6A-0F8C-4151-9C4D-F191CCC4032F
Three species of false click beetles (Coleoptera: Eucnemidae) are added to the Nearctic fauna. One new species, Isorhipis bicolor, is described from a small series collected in Florida, U.S.A. Eighteen specimens collected from Georgia were identified as Dyscharachthis amplicollis (Fleutiaux), new U.S.A. records for a species previously taken from Japan and Southeast Asia. Examination of a series of eucnemids collected by Kyle Schnepp in Florida allowed me to revisit the Deltometopus fauna in eastern North America. Antennal structures present in a series of male specimens in the loan and past examined specimens are definitive enough to resurrect Deltometopus ereptus Bonvouloir, status restored, from synonymy with Deltometopus amoenicornis (Say). Species identification keys are provided for Deltometopus Bonvouloir and Isorhipis Boisduval and Lacordaire in the Nearctic region. Diagnostic differences are briefly noted for each of the three newly added species found in the United States. Images of three species and the related D. amoenicornis are provided.
ZooBank registration. urn:lsid:zoobank.org:pub:30F462F1-966F-4A4F-9D10-BF967AED6574
Inhibitors of the mammalian target of rapamycin (mTOR) have improved the treatment of renal cell carcinoma (RCC). However, chronic drug exposure may trigger resistance, limiting the utility of these agents. The metastatic behavior of RCC cells, susceptible (RCC(par)) or resistant (RCC(res)) to the mTOR inhibitor temsirolimus, was investigated. Adhesion to vascular endothelium or immobilized collagen and fibronectin was quantified. Chemotactic motility was evaluated with a modified Boyden chamber assay. Integrin α and β subtype receptors were analyzed by flow cytometry and Western blot analysis. The physiological relevance of the integrins was then determined by blocking studies and small interfering RNA knockdown. Adhesion to endothelial cells and to fibronectin (not to collagen) and chemotaxis were enhanced in RCC(res) compared to RCC(par). RCC(res) detached from fibronectin and motile activity further increased under retreatment with low-dosed temsirolimus. α5 integrin was diminished inside the cell and at the cell surface, whereas the β3 subtype was reduced intracellularly but elevated at the plasma membrane. In RCC(par), blocking α5 surface receptors enhanced RCC-collagen but reduced RCC-fibronectin interaction, whereas the opposite was true for RCC(res). Chemotaxis of RCC(par) but not of RCC(res) was strongly diminished by the α5 antibody. Blocking β3 significantly lowered chemotaxis with stronger effects on RCC(res), compared to RCC(par). Importantly, β3 knockdown reduced chemotaxis of RCC(par) but upregulated the motile behavior of RCC(res). Temsirolimus resistance is characterized by quantitative alterations of integrin α5 and β3 expression, coupled to functional changes of the integrin molecules, and forces a switch from RCC adhesion to RCC migration.
Human serum albumin (HSA) nanoparticles represent a promising tool for targeted drug delivery to tumor cells. The coupling of the antibody trastuzumab to nanoparticles uses the capability of human epidermal growth factor receptor 2 (HER2)-positive cells to incorporate agents linked to HER2. In our present study, we developed targeted nanoparticles loaded with antisense oligonucleotides (ASOs) against polo-like kinase 1 (Plk1). We evaluated the receptor-mediated uptake into HER2-positive and -negative breast cancer and murine cell lines. We performed quantitative real-time PCR and Western blot analyses to monitor the impact on Plk1 expression in HER2-positive breast cancer cells. Antibody-conjugated nanoparticles showed a specific targeting to HER2-overexpressing cells with cellular uptake by receptor-mediated endocytosis and a release into HER2-positive BT-474 cells. We observed a significant reduction of Plk1 mRNA and protein expression and increased activation of Caspase 3/7. Thus, this is the first report about ASO-loaded HSA nanoparticles, where an impact on gene expression could be observed. The data provide the basis for the further development of carrier systems for Plk1-specific ASOs to reduce off-target effects evoked by systemically administered ASOs and to achieve a better penetration into primary and metastatic target cells. Treatment of tumors using trastuzumab-conjugated ASO-loaded HSA nanoparticles could be a promising approach to reach this goal.
The review of the taxonomic status of Herpetoreas xenura species complex, based on morphological and molecular data, revealed a new species from Myanmar, which we describe as Herpetoreas davidi sp. nov. from the Rakhine Yoma Elephant Wildlife Sanctuary in Rakhine State. It is suggested to be a sister species to Herpetoreas pealii and Herpetoreas xenura sensu stricto, and can be separated from the latter species by a combination of morphological and scalation characters, and by its pattern. Another population from the Htamanthi Wildlife Sanctuary, Sagaing Region, was previously confused with H. xenura. However, as a closer examination of its morphology, shows it to be closer to Hebius khasiensis, we herein tentatively refer to this population as Hebius khasiensis, pending further molecular data confirming its taxonomic status. Consequently, we propose to temporarily remove Herpetoreas xenura from the fauna of Myanmar. Further studies, especially in the northwestern region of Myanmar, such as near border with India in Chin Hills and Naga Hills, are required. An updated key for the species of Herpetoreas is also provided.
Localized prostate cancer exhibits multiple genomic alterations and heterogeneity at the proteomic level. Single-cell technologies capture important cell-to-cell variability responsible for heterogeneity in biomarker expression that may be overlooked when molecular alterations are based on bulk tissue samples. This study aims to identify prognostic biomarkers and describe the heterogeneity of prostate cancer and the associated microenvironment by simultaneously quantifying 36 proteins using single-cell mass cytometry analysis of over 1.6 million cells from 58 men with localized prostate cancer. We perform this task, using a high-dimensional clustering pipeline named Franken to describe subpopulations of immune, stromal, and prostate cells, including changes occurring in tumor tissues and high-grade disease that provide insights into the coordinated progression of prostate cancer. Our results further indicate that men with localized disease already harbor rare subpopulations that typically occur in castration-resistant and metastatic disease.
The cosmopolitan pipunculid genus Dasydorylas Skevington, 2001 includes just a few species from the Neotropical Region and is completely unknown to Colombia. Three new species of Dasydorylas are described from protected areas and conflict territories of limited access in Colombia, namely Dasydorylas colombiensis sp. nov. (type locality: Santuario de Fauna y Flora Iguaque, Boyacá), D. gibber sp. nov. (type locality: Santuario de Fauna y Flora Iguaque, Boyacá), and D. santainesensis sp. nov. (type locality: Páramo de Santa Inés, Belmira, Antioquia). Diagnoses, illustrations and distributional data of the new species are presented. Dasydorylas nigellus (Rafael, 1991) is recorded for the first time from Colombia and its amended diagnosis is provided. An identification key to males of all Neotropical species is presented. With this paper, the number of Neotropical species of Dasydorylas is increased from six to nine.
Objective: To develop a reliable and valid scoring tool, the Pediatric Bowel Management Scoring Tool (PBMST), to better guide management of constipation in pediatric patients.
Study design: The project comprised 2 stages, development of the questionnaire and construction of the bowel management score. Two questionnaires were created, one for children aged 8-18 years to self-report and one parent proxy-report for children aged 4-8 years. Questions regarding physical symptoms (n = 6), emotional aspects (n = 2), social activities/school (n = 1), and treatment (n = 1) were included. Patients (or parents of patients) with symptoms of constipation completed the questionnaire. The reproducibility of each question was computed using the Cohen weighted kappa coefficient (κ). A bowel management score was developed using logistic regression analysis, assessing the associations between the questions and impact on self-reported quality of life (QoL). Questions with adequate reproducibility and significantly associated with QoL were incorporated into the score.
Results: The questionnaire was completed by 385 patients. Six questions met the inclusion criteria and were incorporated into the score: stool shape (range, 0-3 points), anorectal pain (0-4 points), abdominal pain (0-3 points), frequency of fecal incontinence (0-3 points), assistance of caregivers (0-3 points), and interference with social activities (0-6 points). Differences in bowel management scores among patients reporting no, little, some, or major impact on QoL were statistically significant (P < .001).
Conclusions: The newly developed and validated PBMST is a reliable tool for evaluating bowel management strategies in children with constipation.
Highlights
• Cryo-EM structure of a yeast F1Fo-ATP synthase dimer
• Inhibitor-free X-ray structure of the F1 head and rotor complex
• Mechanism of ATP generation by rotary catalysis
• Structural basis of cristae formation in the inner mitochondrial membrane
Summary
We determined the structure of a complete, dimeric F1Fo-ATP synthase from yeast Yarrowia lipolytica mitochondria by a combination of cryo-EM and X-ray crystallography. The final structure resolves 58 of the 60 dimer subunits. Horizontal helices of subunit a in Fo wrap around the c-ring rotor, and a total of six vertical helices assigned to subunits a, b, f, i, and 8 span the membrane. Subunit 8 (A6L in human) is an evolutionary derivative of the bacterial b subunit. On the lumenal membrane surface, subunit f establishes direct contact between the two monomers. Comparison with a cryo-EM map of the F1Fo monomer identifies subunits e and g at the lateral dimer interface. They do not form dimer contacts but enable dimer formation by inducing.