Molecular phylogenetic diversity and biological characterization of diaporthe species associated with leaf spots of camellia sinensis in Taiwan

  • Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.

Download full text files

Export metadata

Author:Hiran A. Ariyawansa, Ichen Tsai, Jian-Yuan Wang, Patchareeya Withee, Medsaii Tanjira, Shiou-Ruei Lin, Nakarin Suwannarach, Jaturong Kumla, Abdallah M. Elgorban, Ratchadawan Cheewangkoon
Parent Title (English):Plants
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/07/14
Date of first Publication:2021/07/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/11/09
Tag:endophytes; foliar pathogens; pathogenicity; taxonomy
Issue:7, art. 1434
Page Number:20
First Page:1
Last Page:20
This study was funded by grants from the Higher Education Sprout Projects (grant number: NTUJP-108L7215 and NTUJP-110L7221) and The Researchers Supporting Project number (RSP-2021/56), King Saud University, Riyadh, Saudi Arabia.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik)
Licence (German):License LogoCreative Commons - Namensnennung 4.0