Age‑dependent gene expression of Calliphora vicina pupae (Diptera: Calliphoridae) at constant and fluctuating temperatures

  • Estimating the age of the developmental stages of the blow fly Calliphora vicina (Diptera: Calliphoridae) is of forensic relevance for the determination of the minimum post-mortem interval (PMImin). Fly eggs and larvae can be aged using anatomical and morphological characters and their modification during development. However, such methods can only hardly be applied for aging fly pupae. Previous study described age estimation of C. vicina pupae using gene expression, but just when reared at constant temperatures, but fluctuating temperatures represent a more realistic scenario at a crime scene. Therefore, age-dependent gene expression of C. vicina pupae were compared at 3 fluctuating and 3 constant temperatures, the latter representing the mean values of the fluctuating profiles. The chosen marker genes showed uniform expression patterns during metamorphosis of C. vicina pupae bred at different temperature conditions (constant or fluctuating) but the same mean temperature (e.g. constant 10 °C vs. fluctuating 5–15 °C). We present an R-based statistical tool, which enables estimation of the age of the examined pupa based on the analysed gene expression data.
Author:Katharina Hartmann, Eva HerrmannORCiDGND, Jens AmendtORCiDGND, Marcel A. VerhoffORCiDGND, Richard ZehnerORCiDGND
Parent Title (English):International journal of legal medicine
Place of publication:Berlin ; Heidelberg
Document Type:Article
Date of Publication (online):2021/09/27
Date of first Publication:2021/09/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/01/12
Tag:Age estimation; Analysis tool; Blow flies; Forensic entomology; Post-mortem interval
Page Number:11
First Page:2625
Last Page:2635
Open Access funding enabled and organized by Projekt DEAL.
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0