Verena Köberle, Thomas Pleli, Christian Schmithals, Eduardo Augusto Alonso, Jörg Haupenthal, Halvard-Björn Bönig, Jan Franz-Josef Peveling-Oberhag, Ricardo M. Biondi, Stefan Zeuzem, Bernd Kronenberger, Oliver Waidmann, Albrecht Piiper
- Background: MicroRNAs circulating in the blood, stabilized by complexation with proteins and/or additionally by encapsulation in lipid vesicles, are currently being evaluated as biomarkers. The consequences of their differential association with lipids/vesicles for their stability and use as biomarkers are largely unexplored and are subject of the present study.
Methods: The levels of a set of selected microRNAs were determined by quantitative reverse-transcription PCR after extraction from sera or vesicle- and non-vesicle fractions prepared from sera. The stability of these microRNAs after incubation with RNase A or RNase inhibitor, an inhibitor of RNase A family enzymes was studied.
Results: The levels of microRNA-1 and microRNA-122, but not those of microRNA-16, microRNA-21 and microRNA-142-3p, declined significantly during a 5-h incubation of the sera. RNase inhibitor prevented the loss of microRNAs in serum as well as the degradation of microRNA-122, a microRNA not expressed in blood cells, in whole blood. Stabilization of microRNA-122 was also achieved by hemolysis. Prolonged incubation of the sera led to enrichment of vesicle-associated relative to non-vesicle-associated microRNAs. Vesicle-associated microRNAs were more resistant to RNase A treatment than the respective microRNAs not associated with vesicles.
Conclusions: Serum microRNAs showed differential stability upon prolonged incubation. RNase inhibitor might be useful to robustly preserve the pattern of cell-free circulating microRNAs. In the case of microRNAs not expressed in blood cells this can also be achieved by hemolysis. Vesicle-associated microRNAs appeared to be more stable than those not associated with vesicles, which might be useful to disclose additional biomarker properties of miRNAs.
MetadatenVerfasserangaben: | Verena KöberleGND, Thomas PleliORCiD, Christian Schmithals, Eduardo Augusto Alonso, Jörg HaupenthalORCiDGND, Halvard-Björn BönigORCiDGND, Jan Franz-Josef Peveling-OberhagORCiDGND, Ricardo M. BiondiORCiDGND, Stefan ZeuzemORCiDGND, Bernd KronenbergerORCiDGND, Oliver WaidmannORCiDGND, Albrecht PiiperORCiD |
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URN: | urn:nbn:de:hebis:30:3-316828 |
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DOI: | https://doi.org/10.1371/journal.pone.0075184 |
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ISSN: | 1932-6203 |
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Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/24073250 |
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Titel des übergeordneten Werkes (Englisch): | PLoS One |
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Verlag: | PLoS |
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Verlagsort: | Lawrence, Kan. |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 20.09.2013 |
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Datum der Erstveröffentlichung: | 20.09.2013 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 25.09.2013 |
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Jahrgang: | 8 |
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Ausgabe / Heft: | (9):e75184 |
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Seitenzahl: | 11 |
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Bemerkung: | Copyright: © 2013 Köberle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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HeBIS-PPN: | 353231886 |
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Institute: | Medizin / Medizin |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |
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