Human mesenchymal stromal cells are resistant to SARS-CoV-2 infection under steady-state, inflammatory conditions and in the presence of SARS-CoV-2-infected cells
- Previous studies reported on the safety and applicability of mesenchymal stem/stromal cells (MSCs) to ameliorate pulmonary inflammation in acute respiratory distress syndrome (ARDS). Thus, multiple clinical trials assessing the potential of MSCs for COVID-19 treatment are underway. Yet, as SARS-inducing coronaviruses infect stem/progenitor cells, it is unclear whether MSCs could be infected by SARS-CoV-2 upon transplantation to COVID-19 patients. We found that MSCs from bone marrow, amniotic fluid, and adipose tissue carry angiotensin-converting enzyme 2 and transmembrane protease serine subtype 2 at low levels on the cell surface under steady-state and inflammatory conditions. We did not observe SARS-CoV-2 infection or replication in MSCs at steady state under inflammatory conditions, or in direct contact with SARS-CoV-2-infected Caco-2 cells. Further, indoleamine 2,3-dioxygenase 1 production in MSCs was not impaired in the presence of SARS-CoV-2. We show that MSCs are resistant to SARS-CoV-2 infection and retain their immunomodulation potential, supporting their potential applicability for COVID-19 treatment.
Verfasserangaben: | Richard SchäferORCiDGND, Gabriele SpohnORCiDGND, Marco Bechtel, Denisa BojkovaORCiDGND, Patrick C. BaerORCiDGND, Selim KuçiORCiDGND, Erhard SeifriedORCiDGND, Sandra CiesekORCiDGND, Jindrich CinatlORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-779592 |
DOI: | https://doi.org/10.1016/j.stemcr.2020.09.003 |
ISSN: | 2213-6711 |
Titel des übergeordneten Werkes (Englisch): | Stem cell reports |
Verlag: | Elsevier |
Verlagsort: | Amsterdam |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 09.03.2021 |
Datum der Erstveröffentlichung: | 11.09.2020 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 13.11.2023 |
Freies Schlagwort / Tag: | ARDS; COVID-19; SARS-CoV-2; inflammation; mesenchymal stromal cells; stem cells |
Jahrgang: | 16.2021 |
Ausgabe / Heft: | 3 |
Seitenzahl: | 9 |
Erste Seite: | 419 |
Letzte Seite: | 427 |
HeBIS-PPN: | 515555010 |
Institute: | Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Lizenz (Deutsch): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |