Sascha W. Weyer, Marta Zagrebelsky, Ulrike Herrmann, Meike Hick, Lennard Ganss, Julia Gobbert, Morna Gruber, Christine Altmann, Martin Korte, Thomas Deller, Ulrike C. Müller
- Synaptic dysfunction and synapse loss are key features of Alzheimer's pathogenesis. Previously, we showed an essential function of APP and APLP2 for synaptic plasticity, learning and memory. Here, we used organotypic hippocampal cultures to investigate the specific role(s) of APP family members and their fragments for dendritic complexity and spine formation of principal neurons within the hippocampus. Whereas CA1 neurons from APLP1-KO or APLP2-KO mice showed normal neuronal morphology and spine density, APP-KO mice revealed a highly reduced dendritic complexity in mid-apical dendrites. Despite unaltered morphology of APLP2-KO neurons, combined APP/APLP2-DKO mutants showed an additional branching defect in proximal apical dendrites, indicating redundancy and a combined function of APP and APLP2 for dendritic architecture. Remarkably, APP-KO neurons showed a pronounced decrease in spine density and reductions in the number of mushroom spines. No further decrease in spine density, however, was detectable in APP/APLP2-DKO mice. Mechanistically, using APPsalpha-KI mice lacking transmembrane APP and expressing solely the secreted APPsalpha fragment we demonstrate that APPsalpha expression alone is sufficient to prevent the defects in spine density observed in APP-KO mice. Collectively, these studies reveal a combined role of APP and APLP2 for dendritic architecture and a unique function of secreted APPs for spine density.
MetadatenAuthor: | Sascha W. Weyer, Marta Zagrebelsky, Ulrike Herrmann, Meike Hick, Lennard Ganss, Julia Gobbert, Morna Gruber, Christine Altmann, Martin Korte, Thomas DellerORCiDGND, Ulrike C. Müller |
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URN: | urn:nbn:de:hebis:30:3-334027 |
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DOI: | https://doi.org/10.1186/2051-5960-2-36 |
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ISSN: | 2051-5960 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24684730 |
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Parent Title (English): | Acta Neuropathologica Communications |
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Publisher: | BioMed Central |
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Place of publication: | London |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2014 |
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Date of first Publication: | 2014/03/31 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2014/04/08 |
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Tag: | Alzheimer; Amyloid precursor protein; Knockout; Neuronal morphology; Spine density |
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Volume: | 2 |
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Issue: | Art. 36 |
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Page Number: | 15 |
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First Page: | 1 |
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Last Page: | 15 |
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Note: | © 2014 Weyer et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
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HeBIS-PPN: | 364444150 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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