Human mesenchymal stromal cells are resistant to SARS-CoV-2 infection under steady-state, inflammatory conditions and in the presence of SARS-CoV-2-infected cells
- Previous studies reported on the safety and applicability of mesenchymal stem/stromal cells (MSCs) to ameliorate pulmonary inflammation in acute respiratory distress syndrome (ARDS). Thus, multiple clinical trials assessing the potential of MSCs for COVID-19 treatment are underway. Yet, as SARS-inducing coronaviruses infect stem/progenitor cells, it is unclear whether MSCs could be infected by SARS-CoV-2 upon transplantation to COVID-19 patients. We found that MSCs from bone marrow, amniotic fluid, and adipose tissue carry angiotensin-converting enzyme 2 and transmembrane protease serine subtype 2 at low levels on the cell surface under steady-state and inflammatory conditions. We did not observe SARS-CoV-2 infection or replication in MSCs at steady state under inflammatory conditions, or in direct contact with SARS-CoV-2-infected Caco-2 cells. Further, indoleamine 2,3-dioxygenase 1 production in MSCs was not impaired in the presence of SARS-CoV-2. We show that MSCs are resistant to SARS-CoV-2 infection and retain their immunomodulation potential, supporting their potential applicability for COVID-19 treatment.
Author: | Richard SchäferORCiDGND, Gabriele SpohnORCiDGND, Marco Bechtel, Denisa BojkovaORCiDGND, Patrick C. BaerORCiDGND, Selim KuçiORCiDGND, Erhard SeifriedORCiDGND, Sandra CiesekORCiDGND, Jindrich CinatlORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-779592 |
DOI: | https://doi.org/10.1016/j.stemcr.2020.09.003 |
ISSN: | 2213-6711 |
Parent Title (English): | Stem cell reports |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/03/09 |
Date of first Publication: | 2020/09/11 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/11/13 |
Tag: | ARDS; COVID-19; SARS-CoV-2; inflammation; mesenchymal stromal cells; stem cells |
Volume: | 16.2021 |
Issue: | 3 |
Page Number: | 9 |
First Page: | 419 |
Last Page: | 427 |
HeBIS-PPN: | 515555010 |
Institutes: | Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |