Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b
- The increased susceptibility to infections of neonates is caused by an immaturity of the immune system as a result of both qualitative and quantitative differences between neonatal and adult immune cells. With respect to B cells, neonatal antibody responses are known to be decreased. Accountable for this is an altered composition of the neonatal B cell compartment towards more immature B cells. However, it remains unclear whether the functionality of individual neonatal B cell subsets is altered as well. In the current study we therefore compared phenotypical and functional characteristics of corresponding neonatal and adult B cell subpopulations. No phenotypic differences could be identified with the exception of higher IgM expression in neonatal B cells. Functional analysis revealed differences in proliferation, survival, and B cell receptor signaling. Most importantly, neonatal B cells showed severely impaired class-switch recombination (CSR) to IgG and IgA. This was associated with increased expression of miR-181b in neonatal B cells. Deficiency of miR-181b resulted in increased CSR. With this, our results highlight intrinsic differences that contribute to weaker B cell antibody responses in newborns.
Author: | Stephanie Gläsener, Christine Jänke, Anika Habener, Robert Geffers, Petra Hagendorff, Katrin Witzlau, Esther Imelmann, Andreas KruegerORCiDGND, Almut Meyer-Bahlburg |
---|---|
URN: | urn:nbn:de:hebis:30:3-456429 |
DOI: | https://doi.org/10.1371/journal.pone.0192230 |
ISSN: | 1932-6203 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/29389970 |
Parent Title (English): | PLoS one |
Publisher: | PloS |
Place of publication: | Lawrence, Kan. |
Contributor(s): | Menno van Zelm |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Date of first Publication: | 2018/02/01 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2018/02/08 |
Tag: | Adults; Antibodies; B cells; Blood; Cell staining; Flow cytometry; MicroRNAs; Neonates |
Volume: | 13 |
Issue: | (2): e0192230 |
Page Number: | 23 |
First Page: | 1 |
Last Page: | 23 |
Note: | Copyright: © 2018 Glaesener et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
HeBIS-PPN: | 426620887 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |