Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
- Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of rectal cancer by intrarectal transplantation of genetically modified organoids into immunocompetent mice followed by precise image-guided radiotherapy (IGRT) of organoid-induced tumors. This model represents a useful platform to study the cellular and molecular determinants of therapy resistance in rectal cancer.
Author: | Adele Mikheal NicolasORCiDGND, Marina PešićGND, Franz RödelORCiDGND, Emmanouil FokasORCiDGND, Florian GretenORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-782987 |
DOI: | https://doi.org/10.1016/j.xpro.2022.101749 |
ISSN: | 2666-1667 |
Parent Title (English): | STAR Protocols |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/10/05 |
Date of first Publication: | 2022/10/05 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/10/18 |
Tag: | Cancer; Health sciences; Model organisms |
Volume: | 3 |
Issue: | 4, 101749 |
Article Number: | 101749 |
Page Number: | 14 |
HeBIS-PPN: | 516756389 |
Institutes: | Medizin |
Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus | |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |