Verena Bihrer, Oliver Waidmann, Mireen Friedrich-Rust, Nicole Forestier, Simone Susser, Jörg Haupenthal, Martin-Walter Welker, Ying Shi, Jan Franz-Josef Peveling-Oberhag, Andreas Polta, Michael von Wagner, Heinfried H. Radeke, Christoph Sarrazin, Jörg Trojan, Stefan Zeuzem, Bernd Kronenberger, Albrecht Piiper
- Background: MicroRNA-21 (miR-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (HCC). Elevated concentrations of miR-21 have also been found in sera or plasma from patients with malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Here we correlated serum miR-21 levels with clinical parameters in patients with different stages of chronic hepatitis C virus infection (CHC) and CHC-associated HCC.
Methodology/Principal Findings: 62 CHC patients, 29 patients with CHC and HCC and 19 healthy controls were prospectively enrolled. RNA was extracted from the sera and miR-21 as well as miR-16 levels were analyzed by quantitative real-time PCR; miR-21 levels (normalized by miR-16) were correlated with standard liver parameters, histological grading and staging of CHC. The data show that serum levels of miR-21 were elevated in patients with CHC compared to healthy controls (P<0.001); there was no difference between serum miR-21 in patients with CHC and CHC-associated HCC. Serum miR-21 levels correlated with histological activity index (HAI) in the liver (r = −0.494, P = 0.00002), alanine aminotransferase (ALT) (r = −0.309, P = 0.007), aspartate aminotransferase (r = −0.495, P = 0.000007), bilirubin (r = −0.362, P = 0.002), international normalized ratio (r = −0.338, P = 0.034) and γ-glutamyltransferase (r = −0.244, P = 0.034). Multivariate analysis revealed that ALT and miR-21 serum levels were independently associated with HAI. At a cut-off dCT of 1.96, miR-21 discriminated between minimal and mild-severe necroinflammation (AUC = 0.758) with a sensitivity of 53.3% and a specificity of 95.2%.
Conclusions/Significance: The serum miR-21 level is a marker for necroinflammatory activity, but does not differ between patients with HCV and HCV-induced HCC.
MetadatenVerfasserangaben: | Verena Bihrer, Oliver WaidmannORCiDGND, Mireen Friedrich-RustORCiDGND, Nicole Forestier, Simone Susser, Jörg HaupenthalORCiDGND, Martin-Walter WelkerGND, Ying Shi, Jan Franz-Josef Peveling-OberhagORCiDGND, Andreas Polta, Michael von Wagner, Heinfried H. RadekeORCiDGND, Christoph SarrazinGND, Jörg TrojanORCiDGND, Stefan ZeuzemORCiDGND, Bernd KronenbergerORCiDGND, Albrecht PiiperORCiD |
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URN: | urn:nbn:de:hebis:30:3-237227 |
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DOI: | https://doi.org/doi:10.1371/journal.pone.0026971 |
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ISSN: | 1932-6203 |
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Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/22066022 |
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Titel des übergeordneten Werkes (Englisch): | PLoS One |
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Verlag: | PLoS |
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Verlagsort: | Lawrence, Kan. |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 31.10.2011 |
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Jahr der Erstveröffentlichung: | 2011 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 27.12.2011 |
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Jahrgang: | 6 |
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Ausgabe / Heft: | 10: e26971 |
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Seitenzahl: | 7 |
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Erste Seite: | 1 |
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Letzte Seite: | 7 |
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HeBIS-PPN: | 287114961 |
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Institute: | Medizin / Medizin |
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| Fachübergreifende Einrichtungen / Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |
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