Igor Tsaur, Anita Thomas, Michelle Monecke, Marion Zugelder, Jochen Rutz, Timothy Grein, Sebastian Maxeiner, Hui Xie, Felix Chun, Florian Rothweiler, Jindrich Cinatl, Martin Michaelis, Axel Haferkamp, Roman A. Blaheta
- Despite recent advances in the treatment of metastatic prostate cancer (PCa), resistance development after taxane treatments is inevitable, necessitating effective options to combat drug resistance. Previous studies indicated antitumoral properties of the natural compound amygdalin. However, whether amygdalin acts on drug-resistant tumor cells remains questionable. An in vitro study was performed to investigate the influence of amygdalin (10 mg/mL) on the growth of a panel of therapy-naïve and docetaxel- or cabazitaxel-resistant PCa cell lines (PC3, DU145, and LNCaP cells). Tumor growth, proliferation, clonal growth, and cell cycle progression were investigated. The cell cycle regulating proteins (phospho)cdk1, (phospho)cdk2, cyclin A, cyclin B, p21, and p27 and the mammalian target of rapamycin (mTOR) pathway proteins (phospho)Akt, (phospho)Raptor, and (phospho)Rictor as well as integrin β1 and the cytoskeletal proteins vimentin, ezrin, talin, and cytokeratin 8/18 were assessed. Furthermore, chemotactic activity and adhesion to extracellular matrix components were analyzed. Amygdalin dose-dependently inhibited tumor growth and reduced tumor clones in all (parental and resistant) PCa cell lines, accompanied by a G0/G1 phase accumulation. Cell cycle regulating proteins were significantly altered by amygdalin. A moderate influence of amygdalin on tumor cell adhesion and chemotaxis was observed as well, paralleled by modifications of cytoskeletal proteins and the integrin β1 expression level. Amygdalin may, therefore, block tumor growth and disseminative characteristics of taxane-resistant PCa cells. Further studies are warranted to determine amygdalin’s value as an antitumor drug.
MetadatenVerfasserangaben: | Igor TsaurORCiDGND, Anita ThomasGND, Michelle Monecke, Marion Zugelder, Jochen RutzGND, Timothy Grein, Sebastian MaxeinerGND, Hui XieORCiDGND, Felix ChunORCiDGND, Florian RothweilerGND, Jindrich CinatlORCiDGND, Martin MichaelisORCiDGND, Axel HaferkampGND, Roman A. BlahetaORCiD |
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URN: | urn:nbn:de:hebis:30:3-714542 |
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DOI: | https://doi.org/10.3390/cancers14133111 |
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ISSN: | 2072-6694 |
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Titel des übergeordneten Werkes (Englisch): | Cancers |
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Verlag: | MDPI |
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Verlagsort: | Basel |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 24.06.2022 |
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Datum der Erstveröffentlichung: | 24.06.2022 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 31.01.2023 |
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Freies Schlagwort / Tag: | amygdalin; cabazitaxel; cabozantinib; complementary/alternative medicine (CAM); docetaxel; prostate cancer; resistant cell lines |
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Jahrgang: | 14 |
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Ausgabe / Heft: | 13, art. 3111 |
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Aufsatznummer: | 3111 |
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Seitenzahl: | 16 |
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Erste Seite: | 1 |
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Letzte Seite: | 16 |
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Bemerkung: | This research was supported by the Brigitta & Norbert Muth Foundation. |
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HeBIS-PPN: | 50538602X |
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Institute: | Medizin |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Lizenz (Deutsch): | Creative Commons - CC BY - Namensnennung 4.0 International |
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