- The sensitive detection of circulating tumour cells in patients with differentiated thyroid cancer may precede the detection of relapse by other diagnostic studies – such as serum thyroglobulin – and thus may have important therapeutic and prognostic implications. We performed reverse transcription-polymerase chain reaction (RT-PCR) on blood samples from patients diagnosed with thyroid disease using two different RT-PCR sensitivities. Additionally, tissue specificity of TG mRNA-expression was determined using RNA extracts from 27 different human tissues. The lower limit of detection was 50–100 TG mRNA producing cells/ml blood using a ‘normal’ RT-PCR sensitivity and 10–20 cells/ml blood using a ‘high’ sensitivity. With the normal sensitivity TG mRNA was detected in 9/13 patients with thyroid cancer and metastasis, 63/137 patients with a history of thyroid cancer and no metastasis, 21/85 with non-malignant thyroid disease and 9/50 controls. With the high sensitivity TG mRNA was detected in 11/13 patients with thyroid cancer and metastasis, 111/137 patients with a history of thyroid cancer and no metastasis, 61/85 with non-malignant thyroid disease and 41/50 controls. Interestingly, using the normal RT-PCR sensitivity TG mRNA transcripts are specific for thyroid tissue and detectable in the peripheral blood of controls and patients with thyroid disease, which correlates with a diagnosis of metastasized thyroid cancer. However, with a high RT-PCR sensitivity, TG mRNA expression was found not to be specific for thyroid tissue and was not correlated with a diagnosis of thyroid cancer in patients. As a consequence, to date TG mRNA detected by RT-PCR in the peripheral blood cannot be recommended as a tumour marker superior to TG serum-level.
MetadatenAuthor: | Jörg BojungaGND, Sandra Johanna Röddiger, Michael Stanisch, Klaus Kusterer, Ralf Kurek, Heiner Renneberg, Stefan Adams, Elmar Lindhorst, Klaus-Henning UsadelGND, Petra-Maria Schumm-DraegerGND |
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URN: | urn:nbn:de:hebis:30:3-457632 |
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DOI: | https://doi.org/10.1054/bjoc.1999.1209 |
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ISSN: | 1532-1827 |
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ISSN: | 0007-0920 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/10817499 |
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Parent Title (English): | British journal of cancer |
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Publisher: | Churchill Livingstone |
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Place of publication: | Edinburgh |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2000 |
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Date of first Publication: | 2000/04/21 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2018/03/05 |
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Tag: | molecular diagnostics; polymerase chain reaction; reverse transcription; thyroglobulin mRNA; thyroid cancer |
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Volume: | 82 |
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Issue: | 10 |
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Page Number: | 6 |
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First Page: | 1650 |
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Last Page: | 1655 |
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Note: | From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
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HeBIS-PPN: | 431861773 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung-Keine kommerzielle Nutzung-Weitergabe unter gleichen Bedingungen |
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