A standard protocol for the analysis of postmortem muscle protein degradation: process optimization and considerations for the application in forensic PMI estimation
- The analysis of postmortem protein degradation has become of large interest for the estimation of the postmortem interval (PMI). Although several techniques have been published in recent years, protein degradation-based techniques still largely did not exceed basic research stages. Reasons include impractical and complex sampling procedures, as well as highly variable protocols in the literature, making it difficult to compare results. Following a three-step procedure, this study aimed to establish an easily replicable standardized procedure for sampling and processing, and further investigated the reliability and limitations for routine application. Initially, sampling and processing were optimized using a rat animal model. In a second step, the possible influences of sample handling and storage on postmortem protein degradation dynamics were assessed on a specifically developed human extracorporeal degradation model. Finally, the practical application was simulated by the collection of tissue in three European forensic institutes and an international transfer to our forensic laboratory, where the samples were processed and analyzed according to the established protocol.
Author: | Stefan PittnerORCiD, Veronika Merold, Sven AndersGND, Larissa LohnerGND, Jens AmendtORCiDGND, Miriam Klinger, Roland HausmannGND, Steffen KißlingGND, Fabio Carlo MonticelliORCiDGND, Janine Geissenberger, Angela Zissler, Peter SteinbacherORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-694603 |
DOI: | https://doi.org/10.1007/s00414-022-02849-3 |
ISSN: | 1437-1596 |
Parent Title (English): | International journal of legal medicine |
Publisher: | Springer |
Place of publication: | Berlin ; Heidelberg |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/06/17 |
Date of first Publication: | 2022/06/17 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/06/02 |
Tag: | Degradation; Muscle; PMI; Protein; Protocol |
Volume: | 136 |
Page Number: | 11 |
First Page: | 1913 |
Last Page: | 1923 |
Note: | Open access funding provided by Austrian Science Fund (FWF). This work was supported by the Austrian Science Fund (FWF), grant P31490. |
Institutes: | Medizin |
Dewey Decimal Classification: | 3 Sozialwissenschaften / 34 Recht / 340 Recht |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |