Darja Karpova, Katrin Dauber, Gabriele Spohn, Doreen Chudziak, Eliza Wiercinska, Miriam Schulz, Allison R. Pettit, Jean-Pierre Levesque, Barbara Romagnoli, Kalpana Patel, Eric Chevalier, Klaus Dembowsky, Halvard-Björn Bönig
- Mobilized blood has supplanted bone marrow (BM) as the primary source of hematopoietic stem cells for autologous and allogeneic stem cell transplantation. Pharmacologically enforced egress of hematopoietic stem cells from BM, or mobilization, has been achieved by directly or indirectly targeting the CXCL12/CXCR4 axis. Shortcomings of the standard mobilizing agent, granulocyte colony-stimulating factor (G-CSF), administered alone or in combination with the only approved CXCR4 antagonist, Plerixafor, continue to fuel the quest for new mobilizing agents. Using Protein Epitope Mimetics technology, a novel peptidic CXCR4 antagonist, POL5551, was developed. In vitro data presented herein indicate high affinity to and specificity for CXCR4. POL5551 exhibited rapid mobilization kinetics and unprecedented efficiency in C57BL/6 mice, exceeding that of Plerixafor and at higher doses also of G-CSF. POL5551-mobilized stem cells demonstrated adequate transplantation properties. In contrast to G-CSF, POL5551 did not induce major morphological changes in the BM of mice. Moreover, we provide evidence of direct POL5551 binding to hematopoietic stem and progenitor cells (HSPCs) in vivo, strengthening the hypothesis that CXCR4 antagonists mediate mobilization by direct targeting of HSPCs. In summary, POL5551 is a potent mobilizing agent for HSPCs in mice with promising therapeutic potential if these data can be orroborated in humans.
MetadatenAuthor: | Darja KarpovaORCiDGND, Katrin Dauber, Gabriele SpohnORCiDGND, Doreen Chudziak, Eliza WiercinskaORCiDGND, Miriam Schulz, Allison R. Pettit, Jean-Pierre Levesque, Barbara Romagnoli, Kalpana Patel, Eric Chevalier, Klaus Dembowsky, Halvard-Björn BönigORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-317183 |
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DOI: | https://doi.org/10.1038/leu.2013.266 |
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ISSN: | 1476-5551 |
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ISSN: | 0887-6294 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24072044 |
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Parent Title (English): | Leukemia |
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Publisher: | Nature Publ. Group |
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Place of publication: | London |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2013/09/27 |
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Date of first Publication: | 2013/09/27 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2013/10/07 |
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Tag: | CXCL12; CXCR4; G-CSF; hematopoietic stem and progenitor cells; mobilization |
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Volume: | 27 |
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Issue: | 12 |
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Page Number: | 10 |
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First Page: | 2322 |
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Last Page: | 2331 |
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Note: | This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
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HeBIS-PPN: | 353305308 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 3.0 |
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