Francesca Prandi, Marco Piola, Monica Soncini, Claudia Colussi, Yuri d' Alessandra, Eleonora Penza, Marco Agrifoglio, Maria Cristina Vinci, Gianluca Polvani, Carlo Gaetano, Gianfranco Beniamino Fiore, Maurizio Pesce
- Saphenous vein graft disease is a timely problem in coronary artery bypass grafting. Indeed, after exposure of the vein to arterial blood flow, a progressive modification in the wall begins, due to proliferation of smooth muscle cells in the intima. As a consequence, the graft progressively occludes and this leads to recurrent ischemia. In the present study we employed a novel ex vivo culture system to assess the biological effects of arterial-like pressure on the human saphenous vein structure and physiology, and to compare the results to those achieved in the presence of a constant low pressure and flow mimicking the physiologic vein perfusion. While under both conditions we found an activation of Matrix Metallo-Proteases 2/9 and of microRNAs-21/146a/221, a specific effect of the arterial-like pressure was observed. This consisted in a marked geometrical remodeling, in the suppression of Tissue Inhibitor of Metallo-Protease-1, in the enhanced expression of TGF-β1 and BMP-2 mRNAs and, finally, in the upregulation of microRNAs-138/200b/200c. In addition, the veins exposed to arterial-like pressure showed an increase in the density of the adventitial vasa vasorum and of cells co-expressing NG2, CD44 and SM22α markers in the adventitia. Cells with nuclear expression of Sox-10, a transcription factor characterizing multipotent vascular stem cells, were finally found in adventitial vessels. Our findings suggest, for the first time, a role of arterial-like wall strain in the activation of pro-pathologic pathways resulting in adventitial vessels growth, activation of vasa vasorum cells, and upregulation of specific gene products associated to vascular remodeling and inflammation.
MetadatenAuthor: | Francesca Prandi, Marco Piola, Monica Soncini, Claudia Colussi, Yuri d' Alessandra, Eleonora Penza, Marco Agrifoglio, Maria Cristina Vinci, Gianluca Polvani, Carlo Gaetano, Gianfranco Beniamino Fiore, Maurizio Pesce |
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URN: | urn:nbn:de:hebis:30:3-371710 |
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DOI: | https://doi.org/10.1371/journal.pone.0117409 |
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ISSN: | 1932-6203 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25689822 |
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Parent Title (English): | PLoS One |
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Publisher: | PLoS |
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Place of publication: | Lawrence, Kan. |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2015/02/17 |
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Date of first Publication: | 2015/02/17 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2015/03/22 |
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Volume: | 10 |
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Issue: | (2): e0117409 |
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Page Number: | 20 |
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Note: | Copyright: © 2015 Prandi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
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HeBIS-PPN: | 368903788 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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