Kim Beyer, Frangis Nikfarjam, Manuel Butting, Markus Meissner, Anke König, Ana Ramirez Bosca, Roland Kaufmann, Detlef Heidemann, August Bernd, Stefan Kippenberger, Nadja Nicole Zöller
- Objective: Curcumin is known for its anti-oxidative, anti-inflammatory and anti-tumorigenic qualities at concentrations ranging from 3.7µg/ml to 55µg/ml. Therefore it is pre-destined for tumour therapy. Due to high oral doses that have to be administered and the low bioavailability of curcumin new therapy concepts have to be developed. One of these therapy concepts is the combination of low curcumin concentrations and UVA or visible light. Aim of our study was to investigate the influence of this treatment regime on oral squamous cell carcinoma cells.
Materials and Methods: A human oral squamous cell carcinoma cell line (HN) was pre-incubated with low curcumin concentrations (0.01µg/ml to 1µg/ml). Thereafter cell cultures were either left un-irradiated or were irradiated either with 1J/cm2 UVA or for 5min with visible light. Quantitative analysis of proliferation, membrane integrity, oxidative potential and DNA fragmentation were done.
Results: It could be shown that low curcumin concentrations neither influenced proliferation, nor cell morphology, nor cell integrity nor apoptosis. When combining these curcumin concentrations with UVA or visible light irradiation cell proliferation as well as development of reactive oxygen species was reduced whereas DNA fragmentation was increased. Concentration as well as light entity specific effects could be observed.
Conclusions: The present findings substantiate the potential of the combination of low curcumin concentrations and light as a new therapeutic concept to increase the efficacy of curcumin in the treatment of cancer of the oral mucosa.
MetadatenAuthor: | Kim Beyer, Frangis Nikfarjam, Manuel Butting, Markus MeissnerORCiDGND, Anke KönigGND, Ana Ramirez Bosca, Roland KaufmannGND, Detlef HeidemannGND, August BerndORCiDGND, Stefan KippenbergerORCiDGND, Nadja Nicole ZöllerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-442514 |
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DOI: | https://doi.org/10.7150/jca.17176 |
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ISSN: | 1837-9664 |
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Parent Title (German): | Journal of cancer |
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Publisher: | Ivyspring Internat. Publ. |
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Place of publication: | Wyoming, NSW |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2017/06/01 |
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Date of first Publication: | 2017/05/11 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2017/06/01 |
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Tag: | UVA; VIS; apoptosis; curcumin; head and neck cancer; proliferation |
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Volume: | 8 |
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Issue: | 7 |
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Page Number: | 13 |
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First Page: | 1271 |
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Last Page: | 1283 |
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Note: | © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
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HeBIS-PPN: | 423588052 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (English): | Creative Commons - Namensnennung-Nicht kommerziell 4.0 |
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