Sandra Rogala, Tamer Ali, Maria-Theodora Melissari, Sandra Währisch, Peggy Schuster, Alexandre Sarre, Thomas Böttger, Eva-Maria Pia Rogg, Jaskiran Kaur, Jaya Krishnan, Stefanie Dimmeler, Samir Ounzain, Thierry Pedrazzini, Bernhard Herrmann, Phillip Grote
- After myocardial infarction in the adult heart the remaining, non-infarcted tissue adapts to compensate the loss of functional tissue. This adaptation requires changes in gene expression networks, which are mostly controlled by transcription regulating proteins. Long non-coding transcripts (lncRNAs) are now recognized for taking part in fine-tuning such gene programs. We identified and characterized the cardiomyocyte specific lncRNA Sweetheart RNA (Swhtr), an approximately 10 kb long transcript divergently expressed from the cardiac core transcription factor coding gene Nkx2-5. We show that Swhtr is dispensable for normal heart development and function, but becomes essential for the tissue adaptation process after myocardial infarction. Re-expressing Swhtr from an exogenous locus rescues the Swhtr null phenotype. Genes depending on Swhtr after cardiac stress are significantly occupied, and therefore most likely regulated by NKX2-5. Our results indicate a synergistic role for Swhtr and the developmentally essential transcription factor NKX2-5 in tissue adaptation after myocardial injury.
MetadatenAuthor: | Sandra RogalaGND, Tamer AliORCiD, Maria-Theodora MelissariORCiD, Sandra Währisch, Peggy Schuster, Alexandre Sarre, Thomas BöttgerORCiD, Eva-Maria Pia RoggGND, Jaskiran KaurGND, Jaya KrishnanORCiD, Stefanie DimmelerORCiDGND, Samir OunzainORCiD, Thierry PedrazziniORCiD, Bernhard HerrmannORCiDGND, Phillip GroteORCiD |
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URN: | urn:nbn:de:hebis:30:3-731231 |
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URL: | https://www.biorxiv.org/content/10.1101/2022.11.14.516395v1 |
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DOI: | https://doi.org/10.1101/2022.11.14.516395 |
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Parent Title (English): | bioRxiv |
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Publisher: | bioRxiv |
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Document Type: | Preprint |
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Language: | English |
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Date of Publication (online): | 2022/11/15 |
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Date of first Publication: | 2022/11/15 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2024/05/24 |
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Tag: | LncRNA; Nkx2-5; hypertrophy; trans |
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Issue: | 2022.11.14.516395 Version 1 |
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Edition: | Version 1 |
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Page Number: | 29 |
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Note: | Now published in Nature Communications doi: 10.1038/s41467-023-42760-y |
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HeBIS-PPN: | 518829634 |
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Institutes: | Medizin |
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| Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
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