Removal of EpCAM-positive tumor cells from blood collected during major oncological surgery using the Catuvab device - a pilot study
- Background: Intraoperative blood salvage (IBS) is regarded as an alternative to allogeneic blood transfusion excluding the risks associated with allogeneic blood. Currently, IBS is generally avoided in tumor surgeries due to concern for potential metastasis caused by residual tumor cells in the erythrocyte concentrate. Methods: The feasibility, efficacy and safety aspects of the new developed Catuvab procedure using the bispecific trifunctional antibody Catumaxomab was investigated in an ex-vivo pilot study in order to remove residual EpCAM positive tumor cells from the autologous erythrocyte concentrates (EC) from various cancer patients, generated by a IBS device. Results: Tumor cells in intraoperative blood were detected in 10 of 16 patient samples in the range of 69–2.6 × 105 but no residual malignant cells in the final erythrocyte concentrates after Catuvab procedure. IL-6 and IL-8 as pro-inflammatory cytokines released during surgery, were lowered in mean 28-fold and 52-fold during the Catuvab procedure, respectively, whereas Catumaxomab antibody was detected in 8 of 16 of the final EC products at a considerable decreased and uncritical residual amount (37 ng in mean). Conclusion: The preliminary study results indicate efficacy and feasibility of the new medical device Catuvab allowing potentially the reinfusion of autologous erythrocyte concentrates (EC) produced by IBS device during oncological high blood loss surgery. An open-label, multicenter clinical study on the removal of EpCAM-positive tumor cells from blood collected during tumor surgery using the Catuvab device is initiated to validate these encouraging results.
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| Author: | Andreas WinterGND, Kai ZacharowskiORCiDGND, Patrick MeybohmORCiDGND, Andreas SchnitzbauerORCiDGND, Peter RufGND, Claudia KellermannGND, Horst LindhoferGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-697878 |
| DOI: | https://doi.org/10.1186/s12871-021-01479-3 |
| ISSN: | 1471-2253 |
| Parent Title (English): | BMC anesthesiology |
| Publisher: | BioMed Central |
| Place of publication: | [Erscheinungsort nicht ermittelbar] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/10/29 |
| Date of first Publication: | 2021/10/29 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/03/12 |
| Tag: | Catumaxomab; EpCAM positive tumor; Intraoperative blood salvage; Leukocyte depletion filter; Tumor cell |
| Volume: | 21.2021 |
| Issue: | art. 261 |
| Article Number: | 261 |
| Page Number: | 11 |
| First Page: | 1 |
| Last Page: | 11 |
| Note: | The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| Note: | The study was funded by LINDIS Blood Care GmbH, Neuendorfstr. 20b, Henningsdorf, Germany. Open Access funding enabled and organized by Projekt DEAL. |
| Note: | Availability of data and materials The datasets analysed are available from the corresponding author upon reasonable request. For data request please contact Andreas Winter, University Hospital Frankfurt, Germany (andreas.winter@kgu.de) |
| Institutes: | Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
