Lactate inhibits the pro-inflammatory response and metabolic reprogramming in murine macrophages in a GPR81-independent manner
- Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
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| Author: | Agustina Errea, Delphine Cayet, Philippe Marchetti, Cong Tang, Jerome Kluza, Stefan OffermannsORCiDGND, Jean-Claude Sirard, Martin Rumbo |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-419969 |
| URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112849 |
| DOI: | https://doi.org/10.1371/journal.pone.0163694 |
| ISSN: | 1932-6203 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27846210 |
| Parent Title (English): | PLoS One |
| Publisher: | PLoS |
| Place of publication: | Lawrence, Kan. |
| Contributor(s): | Jagadeesh Bayry |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2017/01/16 |
| Date of first Publication: | 2016/11/15 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2017/01/16 |
| Volume: | 11 |
| Issue: | (11): e0163694 |
| Page Number: | 11 |
| First Page: | 1 |
| Last Page: | 11 |
| Note: | Copyright: © 2016 Errea et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| HeBIS-PPN: | 402530543 |
| Institutes: | Medizin / Medizin |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
