Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae
- Ribosome biogenesis is essential for cellular function and involves rRNA synthesis, rRNA processing and modification, and ribosomal protein assembly. Ribosome biogenesis factors and small nucleolar RNA assist these events. Ribosomal maturation takes place in the nucleolus, the nucleoplasm, and the cytosol in a coordinated and controlled manner. For example, some ribosomal proteins are thought to be assembled in the cytoplasm based on the observations in Saccharomyces cerevisiae. Here, we used cellular fractionation to demonstrate that cleavage of the 20S intermediate, the precursor to mature 18S rRNA, does not occur in the nucleoplasm of Arabidopsis thaliana. It most likely occurs in the cytoplasm. Further, we verified the proposed localization of RPS10e, RPS26e, and RPL24a/b in the nucleus and RPP1 in the nucleolus of A. thaliana by ribosome profiling, immunofluorescence, and analysis of the localization of GFP fusion proteins. Our results suggest that the order of events during ribosomal protein assembly in the ribosome biogenesis pathway differs between plants and yeast.
Author: | Denise Palm, Deniz StreitGND, Maike Ruprecht, Stefan SimmORCiDGND, Christian Scharf, Enrico SchleiffORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-469372 |
DOI: | https://doi.org/10.1002/2211-5463.12487 |
ISSN: | 2211-5463 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/30186745 |
Parent Title (English): | FEBS Open Bio |
Publisher: | Elsevier on behalf of the Federation of European Biochemical Societies |
Place of publication: | Cambridge |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Year of first Publication: | 2018 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2018/09/11 |
Tag: | Arabidopsis thaliana; eukaryotic system; intracellular protein localization; ribosomal proteins; ribosome biogenesis |
Volume: | 8 |
Issue: | 9 |
Page Number: | 8 |
First Page: | 1437 |
Last Page: | 1444 |
Note: | © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
HeBIS-PPN: | 437851508 |
Institutes: | Biowissenschaften / Biowissenschaften |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Biowissenschaften |
Licence (German): | Creative Commons - Namensnennung 4.0 |