The actin-modulating protein synaptopodin mediates long-term survival of dendritic spines
- Large spines are stable and important for memory trace formation. The majority of large spines also contains synaptopodin (SP), an actin-modulating and plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence of SP within large spines could explain their long lifetime. Indeed, using 2-photon time-lapse imaging of SP-transgenic granule cells in mouse organotypic tissue cultures we found that spines containing SP survived considerably longer than spines of equal size without SP. Of note, SP-positive (SP+) spines that underwent pruning first lost SP before disappearing. Whereas the survival time courses of SP+ spines followed conditional two-stage decay functions, SP-negative (SP-) spines and all spines of SP-deficient animals showed single-phase exponential decays. This was also the case following afferent denervation. These results implicate SP as a major regulator of long-term spine stability: SP clusters stabilize spines, and the presence of SP indicates spines of high stability.
Author: | Kenrick Yap, Alexander Drakew, Dinko SmilovicORCiD, Michael RietscheORCiD, Mandy H. Paul, Mario Vuksic, Domenico Del TurcoORCiDGND, Thomas DellerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-623671 |
DOI: | https://doi.org/10.7554/eLife.62944 |
ISSN: | 2050-084X |
Parent Title (English): | eLife |
Publisher: | eLife Sciences Publications |
Place of publication: | Cambridge |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2020/12/04 |
Date of first Publication: | 2020/12/04 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2021/08/03 |
Volume: | 9 |
Issue: | art. e62944 |
Page Number: | 31 |
First Page: | 1 |
Last Page: | 31 |
HeBIS-PPN: | 486234916 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Medizin |
Licence (German): | Creative Commons - Namensnennung 4.0 |