Marco Reis, Cathrin Jacqueline Czupalla, Nicole Ziegler, Kavi Devraj, Jenny Zinke, Sascha Seidel, Rosario Heck, Sonja Thom, Jadranka Macas, Ernesto Bockamp, Marcus Fruttiger, Makoto Mark Taketo, Stefanie Dimmeler, Karl Plate, Stefan Liebner
- Endothelial Wnt/β-catenin signaling is necessary for angiogenesis of the central nervous system and blood–brain barrier (BBB) differentiation, but its relevance for glioma vascularization is unknown. In this study, we show that doxycycline-dependent Wnt1 expression in subcutaneous and intracranial mouse glioma models induced endothelial Wnt/β-catenin signaling and led to diminished tumor growth, reduced vascular density, and normalized vessels with increased mural cell attachment. These findings were corroborated in GL261 glioma cells intracranially transplanted in mice expressing dominant-active β-catenin specifically in the endothelium. Enforced endothelial β-catenin signaling restored BBB characteristics, whereas inhibition by Dkk1 (Dickkopf-1) had opposing effects. By overactivating the Wnt pathway, we induced the Wnt/β-catenin–Dll4/Notch signaling cascade in tumor endothelia, blocking an angiogenic and favoring a quiescent vascular phenotype, indicated by induction of stalk cell genes. We show that β-catenin transcriptional activity directly regulated endothelial expression of platelet-derived growth factor B (PDGF-B), leading to mural cell recruitment thereby contributing to vascular quiescence and barrier function. We propose that reinforced Wnt/β-catenin signaling leads to inhibition of angiogenesis with normalized and less permeable vessels, which might prove to be a valuable therapeutic target for antiangiogenic and edema glioma therapy.
MetadatenAuthor: | Marco Reis, Cathrin Jacqueline CzupallaORCiDGND, Nicole Ziegler, Kavi DevrajORCiD, Jenny Zinke, Sascha SeidelORCiDGND, Rosario Heck, Sonja Thom, Jadranka Macas, Ernesto Bockamp, Marcus Fruttiger, Makoto Mark Taketo, Stefanie DimmelerORCiDGND, Karl PlateGND, Stefan LiebnerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-323675 |
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DOI: | https://doi.org/10.1084/jem.20111580 |
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ISSN: | 1540-9538 |
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ISSN: | 0022-1007 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/22908324 |
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Parent Title (English): | Journal of experimental medicine |
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Publisher: | Rockefeller Univ. Press |
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Place of publication: | New York, NY |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2013 |
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Date of first Publication: | 2012/08/20 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2013/12/04 |
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Volume: | 209 |
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Issue: | 9 |
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Page Number: | 17 |
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First Page: | 1611 |
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Last Page: | 1627 |
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Note: | Copyright © 2012 Reis et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
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HeBIS-PPN: | 363333568 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung-Keine kommerzielle Nutzung-Weitergabe unter gleichen Bedingungen |
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