Co-expression of CD34, CD90, OV-6 and cell-surface vimentin defines cancer stem cells of hepatoblastoma, which are affected by Hsp90 inhibitor 17-AAG
- Cancer stem cells (CSCs) are nowadays one of the major focuses in tumor research since this subpopulation was revealed to be a great obstacle for successful treatment. The identification of CSCs in pediatric solid tumors harbors major challenges because of the immature character of these tumors. Here, we present CD34, CD90, OV-6 and cell-surface vimentin (csVimentin) as reliable markers to identify CSCs in hepatoblastoma cell lines. We were able to identify CSC characteristics for the subset of CD34+CD90+OV-6+csVimentin+-co-expressing cells, such as pluripotency, self-renewal, increased expression of EMT markers and migration. Treatment with Cisplatin as the standard chemotherapeutic drug in hepatoblastoma therapy further revealed the chemo-resistance of this subset, which is a main characteristic of CSCs. When we treated the cells with the Hsp90 inhibitor 17-AAG, we observed a significant reduction in the CSC subset. With our study, we identified CSCs of hepatoblastoma using CD34, CD90, OV-6 and csVimentin. This set of markers could be helpful to estimate the success of novel therapeutic approaches, as resistant CSCs are responsible for tumor relapses.
Verfasserangaben: | Mieun Lee-TheilenORCiDGND, Julia R. Hadhoud, Giulietta VolanteGND, Delaine D. Fadini, Julia EichhornGND, Udo RolleORCiDGND, Henning C. Fiege |
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URN: | urn:nbn:de:hebis:30:3-634501 |
DOI: | https://doi.org/10.3390/cells10102598 |
ISSN: | 2073-4409 |
Titel des übergeordneten Werkes (Englisch): | Cells |
Verlag: | MDPI |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 29.09.2021 |
Datum der Erstveröffentlichung: | 29.09.2021 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 25.01.2022 |
Freies Schlagwort / Tag: | 17-AAG; CSC marker; Hsp90 inhibitor; cancer stem cells; hepatoblastoma |
Jahrgang: | 10 |
Ausgabe / Heft: | 10, art. 2598 |
Seitenzahl: | 16 |
Erste Seite: | 1 |
Letzte Seite: | 16 |
HeBIS-PPN: | 49129381X |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Medizin |
Lizenz (Deutsch): | Creative Commons - Namensnennung 4.0 |