Intracarotid administration of human bone marrow mononuclear cells in rat photothrombotic ischemia

  • Background: Increasing evidence suggests that cell therapy improves functional recovery in experimental models of stroke and myocardial infarction. So far only small pilot trials tested the effects of cell therapy in stroke patients, whereas large clinical trials were conducted in patients with ischemic heart disease. To investigate the therapeutic benefit of cell therapy to improve the recovery after stroke, we determined the efficacy of bone marrow derived mononuclear cells, which were shown to improve the recovery in experimental and clinical acute myocardial infarction studies, in a rat stroke model. Methods: Adult male Wistar rats were randomly assigned to receive either five million human bone marrow mononuclear cells (hBMC) or placebo intraarterially 3 days after photothrombotic ischemia. For immunosuppression the animals received daily injections of cyclosporine throughout the experiment, commencing 24 hours before the cell transplantation. A battery of behavioural tests was performed before and up to 4 weeks after ischemia. Results: Body temperature and body weight revealed no difference between groups. Neurological deficits measured by the Rotarod test, the adhesive-removal test and the cylinder test were not improved by hBMC transplantation compared to placebo. Conclusions: This study demonstrates that hBMC do not improve functional recovery when transplanted intraaterially 3 days after the onset of focal cerebral ischemia. A possible reason for the failed neurological improvement after cell therapy might be the delayed treatment initiation compared to other experimental stroke studies that showed efficacy of bone marrow mononuclear cells.

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Author:Jens Minnerup, Florian Hartmut SeegerGND, Katharina Kuhnert, Kai Diederich, Matthias Schilling, Stefanie DimmelerORCiDGND, Wolf-Rüdiger Schäbitz
Pubmed Id:
Parent Title (English):Experimental & translational stroke medicine
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Date of Publication (online):2010/02/25
Date of first Publication:2010/02/02
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2010/02/25
Issue:1, Art. 3
Page Number:5
First Page:1
Last Page:5
© 2010 Minnerup et al., licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Experimental & Translational Stroke Medicine 2010, 2:3 ; doi:10.1186/2040-7378-2-3 ;
Institutes:Medizin / Medizin
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Wissenschaftliche Zentren und koordinierte Programme / DFG-Forschergruppen
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0