Region-specific differences in amyloid precursor protein expression in the mouse hippocampus

  • The physiological role of amyloid precursor protein (APP) has been extensively investigated in the rodent hippocampus. Evidence suggests that APP plays a role in synaptic plasticity, dendritic and spine morphogenesis, neuroprotection and—at the behavioral level—hippocampus-dependent forms of learning and memory. Intriguingly, however, studies focusing on the role of APP in synaptic plasticity have reported diverging results and considerable differences in effect size between the dentate gyrus (DG) and area CA1 of the mouse hippocampus. We speculated that regional differences in APP expression could underlie these discrepancies and studied the expression of APP in both regions using immunostaining, in situ hybridization (ISH), and laser microdissection (LMD) in combination with quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting. In sum, our results show that APP is approximately 1.7-fold higher expressed in pyramidal cells of Ammon’s horn than in granule cells of the DG. This regional difference in APP expression may explain why loss-of-function approaches using APP-deficient mice revealed a role for APP in Hebbian plasticity in area CA1, whereas this could not be shown in the DG of the same APP mutants.

Download full text files

Export metadata

Author:Domenico Del Turco, Mandy H. Paul, Jessica Schlaudraff, Meike Hick, Kristina Endres, Ulrike C. Müller, Thomas DellerORCiDGND
Pubmed Id:
Parent Title (English):Frontiers in molecular neuroscience
Publisher:Frontiers Research Foundation
Place of publication:Lausanne
Document Type:Article
Date of Publication (online):2016/12/22
Date of first Publication:2016/11/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/12/23
Tag:APP; CA1; RT-qPCR; dentate gyrus; immunostaining; in situ hybridization; laser microdissection; western blotting
Issue:Article 134
Page Number:12
First Page:1
Last Page:12
Copyright © 2016 Del Turco, Paul, Schlaudraff, Hick, Endres, Müller and Deller. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0