Immunosuppressive compounds affect the fungal growth and viability of defined aspergillus species

  • Immunosuppressive drugs are administered to a number of patients; e.g., to allogeneic hematopoietic stem cell transplant recipients. Immunosuppressive drugs impair the immune system and thus increase the risk of invasive fungal disease, but may exhibit antifungal activity at the same time. We investigated the impact of various concentrations of three commonly used immunosuppressive compounds—cyclosporin A (CsA), methylprednisolone (mPRED), and mycophenolic acid (MPA)—on the growth and viability of five clinically important Aspergillus species. Methods included disc diffusion, optical density of mycelium, and viability assays such as XTT. MPA and CsA had a species-specific and dose-dependent inhibitory effect on the growth of all Aspergillus spp. tested, although growth inhibition by MPA was highest in A. niger, A. flavus and A. brasiliensis. Both agents exhibited species-specific hyphal damage, which was higher when the immunosuppressants were added to growing conidia than to mycelium. In contrast, mPRED increased the growth of A. niger, but had no major impact on the growth and viability of any of the other Aspergillus species tested. Our findings may help to better understand the interaction of drugs with Aspergillus species and ultimately may have an impact on individualizing immunosuppressive therapy.

Download full text files

Export metadata

Author:Stanislaw Schmidt, Michael HogardtORCiDGND, Asuman Demir, Frauke Röger, Thomas LehrnbecherORCiDGND
Pubmed Id:
Parent Title (English):Pathogens
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/11/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/02/10
Tag:Aspergillus species; cyclosporin A; growth inhibition; immunosuppressive agent; methylprednisolone; mycophenolic acid; viability
Issue:4, Art. 273
Page Number:12
First Page:1
Last Page:12
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0